DNA抽提与基因探针标记的几种方法比较

Comparison of different methods for the DNA extraction and probe labelling

比较了苯酚—氯仿、NP—40和盐酸胍3种不同的 DNA 抽提方法,并对基因探针标记同位素掺入法(缺口平移法和随机引物法)和非同位素掺入法在单拷贝基因检测中的应用进行了探讨。

In this paper,we have compared three different methods(phenol-chloro(?)orm;NP-40;Guandium·HCl)for DNA isolation and two different systems(Isotopic andNonisotopic)for probe labelling in the detection of single genomeic copy.