香椿叶总黄酮提取工艺研究

Study on Extraction Process of the Total Flavonoids from Toona sinensis leaves

本文通过水煮、醇沉和有机溶剂萃取等试验建立了一条从香椿叶中提取纯化总黄酮的工艺路线。其中水提的最佳条件为:料液比1:25,微沸2h,提取2次;醇沉的乙醇体积分数为80%,沉淀12h;有机溶剂萃取选用水饱和正丁醇作为萃取剂,萃取2次,萃取率达到93.61%,香椿叶总黄酮纯度为72.3%。本文建立的香椿叶黄酮提取工艺为工业化开发利用香椿黄酮资源奠定了基础,并对其他黄酮资源的提取开发也提供了有益的借鉴和参考。

Objective ： In this study, a new process for extracting and separating total flavonoids from leaves of Toona sinen, sis was presented. Firstly, the water was used to extract total flavonoids from the leaves （ mass of leaves to volume of solvent 1： 25, g/ml; extraction time 2h; extraction twice） and then the distill liquid was deposited by 80% ethanol for 12 hours. And the optimum extraction condition of total flavonoids was water - saturated and n - butanol for two times, the extraction yield reached to 93. 61%. In this paper, the new extractiou process of the total flavouoids from Toona sineruis leaves laid the foundation for the industrial development and utilization, and had also provided beneficial reference to the extraction of other flavonoid