大鼠运动性骨关节损伤模型构建及HWTX-Ⅰ的干预研究

Construction of rat model with athletic joint damage and intervention research of HWTX-Ⅰ

运动性骨关节损伤是一种严重危害运动员运动能力和运动寿命的机械磨损性和无菌慢性进行性骨关节病。长期从事剧烈运动的运动员,如长跑、举重、柔道等项目的运动员,骨关节经常承受过大的压力,更会加速骨关节的磨损,从而导致关节损伤,诱发运动性骨关节炎。一个理想的运动性骨关节损伤动物模型是实验研究能否取得顺利进展的决定性因素之一,而硬脊膜外腔用药是目前临床镇痛及神经保护类药物的常用施药途径。采用通过切断大鼠右膝关节前后交叉韧带结合中等强度运动训练制作大鼠慢性骨关节损伤结合硬脊外腔置管术,构建了重复性好的大鼠运动性骨关节损伤模型。并在此模型上,以Ibuprofen为阳性对照,观察HWTX-Ⅰ硬膜外腔用药对早期(建模14天用药)和晚期(建模28天用药)骨关节运动摩损性病变大鼠骨关节组织形态学改变及血清中SOD和GSH-PX的浓度变化。实验结果显示:HWTX-Ⅰ硬膜外腔用药具有明显抗伤害性作用,能对慢性运动型骨关节损伤病程进展起到缓解和抑制作用,且其效果优于目前临床常用的离子通道选择性低、副作用大的钙通道拮抗剂Ibuprofen,为慢性运动性骨关节病临床抗伤害性药物的研究提供理论和实验依据。

Joint damage is a mechanical abrasion and sterile chronic progressive arthropathy that seriously affect the life of athletes＇ athletic ability and movement.The athltes long been engaged in strenuous exercise,for example long-distance running,weightlifting,judo,have been put too much pressure on the joints.An ideal animal model of athletic joint damage is a decisive factor progressing the experiment research.The epidural administration was currently used in clinical analgesic and neuroprotective in clinic.A reproducible joints injury model of rat by cutting off the right knee after anterior cruciate ligament combined with external cavity catheterization and chronic exercise training was constructed.In this model,Ibuprofen was used as a positive control,the HWTX-Ⅰ epidural medication to the rats,the difference of joint histopathology and SOD and GSH-PX concentration in serum in early（Modeling of 14-day drug） and later（Modeling of 28-day drug） stage were studied.The results showed that HWTX-I epidural administration had obviously antinociceptive efficacy,which could release and inhibit the disease progression of chronic athletic joint damage.The effect was more obvious than calcium channel blocker Ibuprofen which was under clinical use and with lower selection and greater side effects.