HPLC-MS/MS法鉴定落新妇苷在大鼠尿中的代谢产物

Identification of the Major Metabolites of Astilbin in Rat Urine by HPLC-MS/MS

为鉴定大鼠给药落新妇苷后尿中的主要代谢产物,收集其尿样,用聚酰胺固相萃取小柱处理后进样,采用高效液相色谱-串联质谱(HPLC-MS/MS)法分析。电喷雾离子源在负离子模式下进行扫描,借助碰撞诱导解离方式进行落新妇苷在大鼠体内主要代谢产物的结构确证。共鉴定出6个落新妇苷在大鼠尿中的代谢产物,分别为落新妇苷异构体(M1)、3′-O-甲基化落新妇苷(M2)、3′-O-甲基化落新妇苷异构体(M3)、槲皮素-鼠李糖苷(M4)、葡萄糖醛酸化落新妇苷(M5)、3′-O-甲基化落新妇苷-葡萄糖醛酸化物(M6)。该方法对落新妇苷在大鼠体内的代谢进行了初步研究,并报道了落新妇苷在大鼠尿中的另外4个代谢产物M1、M3、M4、M6。

To identify the major metabolites of astilbin in rats, urine samples were collected after intragastric administration of astilbin. The urine samples were pretreated by solidphase extraction using polyamide cartridges, and then were analyzed by high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）. Electrospray ionization source operated in negative mode for scan and collision induced dissociation were used to elucidate the structures of the major metabolites of astilbin. The data indicated that the main urine metabolites of astilbin are stereoisomers of astilbin （M1）, 3＇-O-methylastilbin （M2）, a stereoisomer of 3＇-O-methylastilbin （M3）, quercetin rhamnoside （M4）, astilbin glucuronide （M5）, 3＇-O-methylastilbin glucuronide （M6）. Metabolites of M1, M3, M4 and M6 are reported from rat urine.