人与大鼠皮下脂肪细胞分解的受体调节差异研究

STUDIES ON THE DIFFRENCE OF RECEPTOR REGULATION IN LIPOLYSIS OF ABDOMINAL SUBCUTANEOUS LIPOSE CELLS BETWEEN HUMAN AND RAT

用不同的选择性 β受体激动剂分别作用于人和大鼠脂肪细胞，用放射免疫法测定 ｃ Ａ Ｍ Ｐ浓度，并观察不同的选择性 β受体阻断剂对激动剂阻断作用的强度。结果表明，选择性 β３ 受体激动剂作用于大鼠脂肪细胞后能产生 ８３２μｍ ｏｌ／ Ｌ 的ｃ Ａ Ｍ Ｐ，作用人脂肪细胞后仅能产生 １４０μｍ ｏｌ／ Ｌ 的ｃ Ａ Ｍ Ｐ。选择性 β１和 β２ 受体阻断剂阻断异丙肾上腺素对大鼠脂肪细胞的激动作用较弱（分别为 １５％ 和 ２７％ ），选择性 β２ 受体阻断剂约能阻断异丙肾上腺素对人脂肪细胞作用的 ６５％ 。提示大鼠脂肪细胞分解主要由 β３ 受体调节，人则主要由 β２ 受体调节。

In order to explore the difference of receptor regulation in lipolysis of abdominal subcutaneous lipose cells between human and rat, the selectivity β 3 adrenoceptor agonist (BRL 37344 )was used to activize the β adrenoceptor on lipose cells of human and rat respectively, and to determine the cAMP concentration by radioimmunoassay. The selectivity β 1 and β 2 receptor antagonists (Atenolol and ICI 118,551 ) were used to antagonize the effect of isoprenaline, and to study the antagonisted intensity. The results showed that BRL 37344 could produce 8.32mol/L cAMP after acting the lipose cells of rat, but it only produce 1.40mol/L cAMP after acting the human lipose cells. Both Atenolol and ICI 118,551 blocking the effect of iosprenaline were weaker(maximum inhibition 15% and 27%) in lipose cells of rat. The ICI 118,511 ,could block about 65% effect of isoprenaline in human lipose cells. These results indicate that the lipolysis of abdominal subcutenous lipose cells is mediated by β 3 adrenoreceptor in rat, but by β 2 adrenoceptor in human being.