雷公藤多甙对肾性蛋白尿患者外周血单个核细胞作用的研究

THE STUDY ON EFFECT OF TRIPTERYGIUM WILFORDII GLYCOSIS BY PERIPHERAL BLOOD MONONUCLEAR CELLS FROM PROTEINURIC PATIENTS

通过琼脂糖凝胶电泳， Ｈ Ｅ 染色和流式细胞仪检测用雷公藤多甙治疗肾性蛋白尿患者 ２ 周后外周血单个核细胞的凋亡情况。经治疗后患者外周血单个核细胞在形态上出现典型的细胞核固缩、碎裂，琼脂糖凝胶电泳显现特征性的“梯状”带，流式细胞仪上出现亚二倍体峰。提示雷公藤多甙能诱导肾性蛋白尿患者外周血单个核细胞的凋亡。

To evaluate the role of apoptosis on the pharmacologic effects of tripterygium wilfordii glycosides (TWG) in proteinuric patients. Apoptosis of peripheral blood mononuclear (PBMC) was detected by gel electrophoresis, HE staining and flow cytometry. After treatment by TWG, there were typical nuclear condensation and fragments, “DNA ladder” was viewed on agarose gel,“Sub G 1”peak was recorded in DNA histogram of flow cytometry at two weeks. These results suggest that TWG used to treat proteinuric patients can induce apoptotic of PBMC and may be one of the mechanisms for inhibition.