摘要
以光滑球拟酵母(Torulopsis glabrata)HB20为出发菌,经过UV和DES复合诱变选育出一株丙酮酸高产菌HB304。鉴定其为维生素营养缺陷型,摇瓶发酵产酸量比原菌增加了31.2%;优化培养基后其摇瓶发酵产酸量比原菌增加了41.9%。
A high pyruvic acid-producing strain HB304 was bred from Torulopsis glabrata HB20 by UV-DES compound mutation.The strain HB304 appeared to be auxotroph.The yield of pyruvic acid was increased by 31.2% than that of the wild strain in flask culture.After optimization about the medium,the yield of pyruvic acid was increased by 41.9% than that of the wild strain in flask culture.
出处
《化学与生物工程》
CAS
2011年第2期59-62,共4页
Chemistry & Bioengineering
基金
湖北工业大学发酵工程省部共建教育部重点实验室2009年开放基金课题(2009KFJJ07)
关键词
丙酮酸
复合诱变
育种
优化
pyruvic acid
compound mutation
breeding
optimization