乙型肝炎患者血清IL-16含量与其抗原抗体模式及HBV-DNA载量的关系研究

Relationship between serum IL-16 level with antigen-antibody models and HBV DNA load in patients with hepatitis B

目的探索乙型肝炎患者抗原抗体模式、HBV-DNA载量与血清白细胞介素-16(IL-16)含量的相关性,为乙型肝炎发病机制的研究提供新的思路。方法用时间分辨荧光分析法(TRFIR)检测乙型肝炎血清标志物(HBsAg、抗-HBs、HBeAg、抗-HBe及抗-HBc IgG);用实时荧光定量聚合酶链反应(FQ-PCR)法检测HBV-DNA;用酶联免疫吸附法(ELISA)测定IL-16。结果乙型肝炎患者血清中IL-16含量与健康对照相比明显升高(P<0.01),并且1、3模式及1、3、5模式的患者血清中IL-16含量与1、4、5模式相比明显升高(P<0.01);HBV-DNA载量大于或等于500与HBV-DNA载量小于500的乙型肝炎患者血清IL-16水平相比明显升高(P<0.01)。结论血清IL-16在HBV感染早期病毒复制期明显升高,提示IL-16可能参与HBV感染后急性期的肝损伤过程,是一种潜在的乙型肝炎早期促炎性反应细胞因子。

Objective To investigate the correlation between the models of antigen-antibody,HBV DNA load in the patients with hepatitis B and the serum levels of interleukin-16 to provide a new thinking for studying the pathogenesis of hepatitis B.Methods TRFIA was used to determine HBsAg,anti-HBs,HBeAg,anti-HBe and anti-HBc IgM.FQ-PCR was used to measure HBV DNA.ELISA was applied to detect the levels of interleukin-16 in serum.Results The levels of IL-16 in both the models of antigen-antibody and the load of HBV DNA in the patients infected with HBV were much higher than those in healthy control group,respectively with statistic difference between 1,3/1,3,5 models and 1,4,5 models,between HBV DNA positive group and negative group.Conclusion Serum IL-16 level is obviously increased in both early hepatitis B and period with a good many HBV DNA virus reproduction,which shows that IL-16 might participate in the course of liver injury in acute and severe hepatitis B and IL-16 is a potential cytokine for promoting inflammation.