人胃癌细胞腹膜粘附影响因素的实验研究

Experimental study of adhesion of gastric cancer cells to the peritoneum

目的 探讨影响人胃癌细胞在腹膜粘附的因素，为临床上采取有效措施，减少腹膜转移的发生提供依据。方法 用人胃癌细胞株在小鼠腹膜上培养，以ＭＴＴ法检测粘附细胞数的体外实验模型，观察腹膜损伤、血液成分和癌胚抗原（ＣＥＡ）对胃癌细胞在腹膜粘附的影响。结果 腹膜损伤组的粘附细胞数（ＡＤ） 较对照组明显增高（ Ｐ＜ ０－０１） 。血液中的白细胞、血小板组ＡＤ值较对照组明显增高（Ｐ＜０－０１） ，血浆组ＡＤ值亦较对照组增高（ Ｐ＜ ０－０５）。ＣＥＡ组ＡＤ值明显高于对照组（ Ｐ＜ ０－０５）。结论 腹膜损伤、血液成分中的白细胞、血小板、血浆以及ＣＥＡ，均可增加癌细胞在腹膜的粘附。减少腹膜损伤，减少血液中的粘附因子及ＣＥＡ在腹腔滞留机会。

Objective To identify factors in favor of tumor cell adherence to the peritoneum.Methods Pieces of peritoneum from BALB/c mice were collected and incubated in vitro in the presence of human gastric cancer MGC 803 cells. Cells adhered to the peritoneum was assessed by MTT staining. The result was expressed as the percentage of tumor cells adhered (AD).Results If the peritondum had been previously injured, either by scraping or exposure to air, AD was significantly more than that of the control ( P <0.01). AD was significantly increased as compared to the control when the peritoneum was cultured in the presence of white cells, platelets, plasma or CEA.Conclusion Adhesion of cancer cells to the peritoneum may be facilitated by peritoneal injury, and the presence of normal blood constituents. Oncofetal antigen with adhesive property such as CEA also helps tumor cells adhere to the peritoneum. The likelihood of post operative peritoneal metastasis can be minimized if care has been taken to protect the peritoneum from injury and to thoroughly wash the peritoneal cavity before the abdomen is closed.