杏鲍菇和乳白耙齿菌锰过氧化物酶活性规律的研究

Detection on Manganese Peroxidase of Pleurotus eryngii and Irpex lacteus

采用LNAS培养基在4种不同培养液中,对白腐菌杏鲍菇和乳白耙齿菌在28℃下进行静止培养,得到了不同时间内的培养液,用紫外-可见分光光度计检测了在470 nm处对于2,6-DMP的氧化作用后光密度值的变化情况,作为锰过氧化物酶(MnP)产生和活性大小的依据,从而获得了杏鲍菇和乳白耙齿菌锰过氧化物酶与培养基的成分和酶作用底物的关系。结果表明,杏鲍菇和乳白耙齿菌均可产生MnP,对比4种成分不同的培养基酶活力测定结果,在培养液内添加酶的底物木屑和2,6-DMP后可提高MnP的分泌量。以前的研究认为:Mn2+是MnP产生的必要因子。而本试验研究结果表明,Mn2+并不是杏鲍菇和乳白耙齿菌产生MnP所必需的。论文为进一步利用这两种真菌产木质素降解酶,以及为进一步深入研究这两种真菌MnP对木质素及其它异生物质降解的作用机理提供基础的酶学研究。

White rot fungi Pleurotus eryngii and Irpex lacteus were stilly cultured at 28℃ in four different kinds of LNAS culture solution,the extracellular enzyme solutions were sampled at different interval,O.D.values of the solutions,representing manganese peroxidase（MnP） activities,were measured spectrophotometrically by monitoring the oxidation of 2,6-DMP at 470nm,the manganese peroxidase activity of the fungi and the relationships between enzyme activity and medium composition and substrates were gained.Results indicated that P.eryngii and I.lacteus both could produce MnP.Substrates wood sawdust and 2,6-DMP could enhance MnP activity.Our work revealed that Mn2＋ was dispensable for the production of MnP.,which was different from the previous study.The paper offered a basic ligninolytic enzymology of P.eryngii and I.lacteus for future utilization of the enzyme and further study on MnP mechanism of the fungi.