一株嗜盐菌新种的分离与鉴定

Seperation and Identification of a Novel Halophile

从舟山册子岛船舶压载水泥样中分离到一株细菌S3-22,其与已知细菌的16S rDNA序列相似性低于97%,G+C mol%为54.9 mol%,主要脂肪酸iso-C17:1ω9c(24.99%),细胞醌型为甲基萘醌MK-5。革兰氏染色阴性,最适生长条件为30～37℃、pH7、3%NaCl。嗜盐,氧化酶、接触酶、淀粉酶、酯酶呈阳性,可还原硝酸盐。依据其16S rDNA序列相似性、系统发育学分析及细胞与分子水平的鉴定表明,该菌是Kordiimonas属的一个新种,菌株S3-22的16S rDNA序列登陆号为FJ847942。

A marine bacterium ,designated strain S3-22 ,was isolated from the ballast water sample from Zhoushan archipelago,China. 16S rDNA sequences similarity of strain S3-22 to all recognized bacterial species was not greater than 97%. The DNA G ＋ C content was 54.9 mol%. The dominant fatty acid was iso-C17 ： 1ω 9c （24.99%）. The major respiratory quinine was MK-5. Cells of strain S3-22 were gram-negative. Optimal growth of strain S3-22 was at pH 7.0,30 - 37 ℃ and 3 % （w/v） NaCl. It was halophilic and tested positive for catalase, oxidase, amylase, and esterase. In addition,it could deoxidize the nitrate. Based on the evidence of 16S rDNA sequences similarities, phylogenetic analysis and cellular and molecular level identification, the halophile formed a phyletic lineage which was distinct from the known Kordiimonas species. Strain $3-22 represented a novel species in the Kordiimonas, its Genebank Accession number was FJ847942.