微波协同酶法提取平菇柄多糖的工艺研究

Extract Technology of Polysaccharide from Pleurotus ostreatus Stem by Microwave-enzyme Synergistic Method

为优化微波协同酶法提取平菇柄多糖的工艺条件,在单因素试验的基础上,选择提取时间、微波功率以及液料比为自变量,多糖得率为响应值,采用中心组合设计的方法,研究各自变量及其交互作用对多糖得率的影响。利用响应面分析方法,模拟得到二次多项式回归方程的预测模型,并确定平菇柄多糖提取工艺的最佳条件为:微波功率420 W,提取时间8 min,液料比55:1,在此条件下,最大得率达到6.05%。

To optimize the microwave-enzyme synergistic extraction process of Pleurotus ostreatus stem polysaccharides, on the base of single factor investigation,effects of extraction time,microwave power,and ratio of solvent to material as well as their interactions on yield of the polysaccharides were studied by central composite design. The predictive polyno- mial quadratic equations model was developed by response surface analysis. The optimal extraction conditions of the P. ostreatus stem polysaccharides concluded as follows ： extraction time 8 win, micro.wave power 420 W, and ratio of water to material 55： 1. Under these conditions ,the yield of the polysaccharides was up to 6.05%.