丙烯酰胺对大鼠运动功能和学习记忆能力的影响

Effects of Acrylamide on Motor Function and Learning-Memory of Rats

目的观察丙烯酰胺(acrylamide,ACR)亚慢性染毒对大鼠运动功能和学习记忆能力的影响。方法将27只健康清洁级Wistar雄性大鼠随机分为对照组(生理盐水)和低(20 mg/kg)、高剂量(40 mg/kg)ACR染毒组,每组9只。采用腹腔注射方式进行染毒,染毒容量为2 ml/kg,每周染毒3次,连续染毒8周。每周测量大鼠体重,进行步态评分、负重游泳试验、握力试验和水迷宫试验。结果丙烯酰胺染毒可引起大鼠体重减轻(P<0.05),步态评分增高(P<0.05),负重游泳试验时间缩短(P<0.05),握力试验时间缩短(P<0.05),水迷宫试验潜伏期延长(P<0.05)和游泳路程增加(P<0.05)。结论丙烯酰胺可引起大鼠运动功能和学习记忆能力降低。

Objective To study the effect of acrylamide by subchronic poisoning on motor function and learning-memory capability of rats. Methods Thirty male Wistar rats weighing 180-220 g were randomly divided into three groups, 10 in each. The rats of experimental groups were given aerylamide dissolved in normal saline through intraperitoneal injection, at the doses of 20,40 mg/kg, 3 days/week, for 8 weeks. The rats of control group were given normal saline. Motor function indexes were determined per week. Special tests included observations of spontaneous locomotion in an open field, measures of swim and grip time. Learning and memory ability was tested by Morris water maze （MWM）. Results The weight of rats treated with acrylamide was decreased （P〈0.05）. Gait score of rats in high-dose group was increased. Compared with the control group, swim time of rats in low-dose and high-dose group was significantly decreased by 63% and 92% respectively. Grip time in grip test in low-dose and high-dose group were significantly decreased by 47% and 91% respectively. Between ACR and the control groups there were significant differences in MWM （P〈0.05）,the latency at high-dose group was extended by 168% and the distance was increased by 110%. Conclusion Acrylamide can damage motor function and learning-memory capability of rats.