纳米四氧化三铁对小鼠外周血象的影响

Effect of Nano-Fe_3O_4 on Hematological System in Mice

目的观察纳米四氧化三铁颗粒(nano-Fe3O4)对小鼠外周血象的影响。方法将72只成年雄性清洁级ICR小鼠随机分为4组,分别为溶剂对照(高纯水)组和低剂量(10 mg/kg,1/16 LD50)、中剂量(20 mg/kg,1/8 LD50)和高剂量(80 mg/kg,1/2 LD50)nano-Fe3O4染毒组,每组6只。采用一次性尾静脉注射方式进行染毒,注射容积为10 ml/kg,注射速度约为0.02 ml/s。染毒后第1、3、7天,检测全血中白细胞(WBC)、红细胞(RBC)、血小板(PLT)计数及血红蛋白(HGB)含量的变化。结果全血中WBC计数在染毒后第1天,各剂量组未见明显差异(P>0.05);染毒后第3天,中、高剂量组明显低于溶剂对照组和低剂量组(P<0.05),且高剂量组明显低于中剂量组(P<0.05),而低剂量组与溶剂对照组无明显差异(P>0.05);染毒后第7天,各剂量组均明显低于溶剂对照组(P<0.05)。RBC计数及HGB含量在染毒后1天,各剂量组均明显低于溶剂对照组(P<0.05);染毒后第3天逐渐回升(P>0.05);染毒后第7天,中、高剂量组明显高于溶剂对照组和低剂量组(P<0.05)。PLT计数在染毒后第1天,高剂量组明显低于中、低剂量组和溶剂对照组(P<0.05);染毒后第3天,各剂量组均明显低于溶剂对照组(P<0.05);染毒后第7天,高剂量组又明显低于中、低剂量组和溶剂对照组(P<0.05)。结论在本实验条件下,一次性尾静脉注射nano-Fe3O4可使小鼠全血中WBC和PLT计数下降,RBC计数和HGB含量上升。

Objective To observe the effect of nano-Fe3O4 on hematological system in mice. Methods Seventy-two adult male ICR mice were randomly divided into four groups：control group （high pure water）,low （10 mg/kg, 1/16 LD50）,moderate （20 mg/kg, 1/8 LD500）,high （80 mg/kg, 1/2 LD50） dose group of nano-Fe3O4,6 mice in each group. Nano-Fe3O4 was given through tail vein injection, the volume of injection was 10 ml/kg,the injection rate was about 0.02 ml/s. The contents of WBC ,RBC,HGB and PLT change in blood were determined on 1,3,7 day after injection, Results No significant difference was seen in the count of WBC between the treatment groups and control group after 1 day; for moderate and high dose group, it was significantly lower compared with control group and low dose group after 3 day （P〈0.05）; and the high dose group was lower significantly than the moderate dose group （P〈0.05）; after 7 days, the treatment groups were significantly lower than that in control group（P〈0.05 ）. The counts of RBC in the treatment groups were significantly lower than that in control group after one day （P〈0.05）;after 3 days, they increased gradually; after 7 days,in high and moderate dose group they were significantly higher those in low dose group and control group （P〈0.05）. The change of HGB was similar with the change of RBC after injected nano-Fe3O4. The content of PLT in high dose group were significantly lower than that in control group after one day; after 3 days,in the treatment groups they were significantly lower than that in control group （P〈0.05）; after 7 days,the high dose group it was significantly lower compared with the other groups（P〈0.05 ）. Conclusion In the experimental condition, nano-Fe3O4 may have an adverse effect on mice hemogram.