灭幼脲缓释微胶囊的制备与性能

Preparation and Properties of Chlorbenzuron Microcapsules in Sustained Release

[目的]通过对灭幼脲缓释微胶囊的制备和对其性能的测试来提高灭幼脲的稳定性与环境的相容性。[方法]采用壳聚糖和海藻酸钠作为囊壁材料,利用静电吸附层层自组装技术(Layer-by-Layer,LbL法)制备灭幼脲微胶囊。正交优化灭幼脲微胶囊制备工艺,利用扫描电子显微镜和激光共聚焦显微镜表征微胶囊表面结构,研究了微胶囊的体外释放行为。[结果]实验结果表明:分别加入1 mL海藻酸钠(1.0 g/L)、1 mL壳聚糖(1.0 g/L)、20 mg灭幼脲、1 mL氯化钙(1.0 g/L)能得到相对更好的结果。正交试验4个因素中,氯化钙质量浓度对评估结果影响最大;利用优化后的体系制备的灭幼脲微胶囊,平均粒径为10μm,Zeta电位为+23.5 mV;载药量和包封率分别为(68.8±0.86)%和(69.1±0.86)%。[结论]利用这种方法制备的灭幼脲微胶囊具备明显的缓释性能。

[Aims]The aim is to improve the stability of chlorbenzuron（CBZ） and the compatibility with the environment.[Methods] Chlorbenzuron microcapsules were encapsulated with nature chitosan（CHI） and sodium alginate（ALG） by Layer-by-Layer（LbL） self-assembly.The preparation of the CBZ microcapsules was optimized using the orthogonal experiments.The coated colloids were characterized using confocal laser scanning microscopy（CLSM） and scanning electron microscopy（SEM）.The in vitro release pattern of CBZ through the polyelectrolyte diffusion barrier was studied.[Results] The results showed that we could get better result with relative additions of 1 mL alginate（1.0 g/L）,1 mL chitosan（1.0 g/L）,20 mg CBZ,1 mL CaCl2（1.0 g/L）,and we could conclude that the assessment result was affected most by the concentration of CaCl2 among those 4 elements of the orthogonal experiment.For the CBZ microcapsules prepared with the optimized system,the mean grain size was 10 μm,the Zeta electric potential was ＋23.5 mV;the drug loading and encapsulation efficiency were（68.8±0.86）% and（69.1±0.86）% respectively.[Conclusions] The CBZ microcapsules prepared by this method possessed the conspicuous controlled-release property.