2株红树林淤泥放线菌的鉴定及抗菌活性研究

Appraisal and antibiotic activity research on two mangrove forest silt ray fungi

【目的】对分离自广西北海红树林海洋淤泥中的2株放线菌(BH200951、BH200954)进行鉴定,并对其抗菌活性进行分析。【方法】对BH200951与BH200954 2株典型放线菌的形态特征及生理生化特征进行了观测,并采用医学药敏试验方法对其抗菌活性进行了测定,最后提取2株菌株的总DNA,用放线菌通用引物对其16 SrDNA进行PCR扩增,对获得的扩增结果进行DNA序列测定,采用邻接法构建其与其他典型菌株的系统发育树。【结果】在高氏1号培养基上,BH200951和BH200954菌株生长良好,菌落分别呈灰白色和黄色,菌落皱褶,致密坚实;在光学显微镜下观察发现,2株菌株气生菌丝发达,呈深灰色,成熟后生成孢子丝;能使明胶液化,淀粉、纤维素水解,牛奶凝固而不胨化;对金黄色葡萄球菌、表皮葡萄球菌、大肠埃希菌、普通变形杆菌均有较好的抑制作用。经测序后比对显示,2株放线菌菌株均属于链霉菌属,其中BH200951鉴定为链霉菌属(Streptomyces aurantiogriseus)AY999773的变种;BH200954初步鉴定为链霉菌属的仙台链霉菌。【结论】对分离自广西北海红树林海洋淤泥放线菌BH200951和BH200954菌株进行了鉴定,其发酵产物具有明显的抗菌活性。

【Objective】 Two actinomycetes（BH200951,BH200954） isolated from the marine mangrove mud Beihai were identified,and their antimicrobial activity was analyzed.【Method】 Typical morphological,physiological and biochemical characteristics of BH200951 and BH200954 actinomycetes were observed,and susceptibility testing was performed to determine their antibacterial activity.The final total DNA strains was extracted,with the released strain 16S rDNA amplified,and the PCR amplification results was sequenced,the phylogenetic tree was constructed using the neighboring method.【Result】 Gao 1 medium,BH200951 and BH200954 strains grew well,colony color was gray and yellow,respectively,wrinkled colonies,dense solid;optical microscope showed that two strains of aerial hyphae developed,dark gray,mature growth of Sporothrix;gelatin liquefaction could be made,starch cellulose hydrolysed,milk solidified,but not frozen;against Staphylococcus aureus,Staphylococcus epidermidis,Escherichia coli,Proteus vulgaris had good inhibition.Two actinomycete strains were sequenced and compared to be genus Streptomyces,of which BH200951 was identified as Streptomyces（Streptomyces aurantiogriseus） AY999773 variants;BH200954 identified as Streptomyces genus Streptomyces Sendai.【Conclusion】 Isolated from marine mud in Beihai,Guangxi Mangrove actinomycetes strains BH200951 and BH200954 were identified,and its fermentation products showed significant antibacterial activity.