海藻酸钠固定化β-葡萄糖醛酸苷酶的研究

Study on the immobilization of β-glucuronidase by sodium alginate

本文研究了以海藻酸钠为载体,采用交联-包埋方式固定β-葡萄糖醛酸苷酶的固定化工艺。分别考察海藻酸钠浓度、戊二醛体积分数、氯化钙浓度、交联时间和固化时间对固定化酶相对酶活力的影响,并以正交试验确定β-葡萄糖醛酸苷酶最佳的固定化条件:海藻酸钠质量浓度35g/L、给酶量1600U/g载体、戊二醛体积分数0.2%、氯化钙质量浓度20g/L、交联时间2h、固化时间2h。研究表明此时固定化酶的回收率较高,可达到71.66%,本文使用的固定化工艺简单易行,具有广阔的工业前景。

β-glucuronidase was immobilized by sodium alginate as the carrier.The method of cross-linking-embedding was adopted to immobilize β-glucuronidase.The effects of reaction conditions（the concentration of sodium alginate,glutaraldehyde concentration,CaCl2concentration,cross-linking time and immobilized time）on the relative activity of immobilized β-glucuronidase were tested.The orthogonal test was used to investigate the optimal condition of immobilization.The results showed that the best efficiency of immobilization was obtained when sodium alginate concentration was 35 g/L,β-glucuronidase dosage was 1600 U,glutaraldehyde concentration was 0.2%,CaCl2 concentration was 20g/L,and immobilized time was 2h.The recovery rate of immobilized enzyme activity was 71.66 %.The study showed that this immobilize method was simple and had a prospective industrial application.