小儿肺炎支原体检测临床流行病学分析

Study of Clinical Epidemiology on Children's Infectious diseaae caused by MP with IFA

应用美国Ｚｅｕｓ Ｓｃｉｅｎｔｉｆｉｃ Ｉｎｃ 公司提供的肺炎支原体间接免疫荧光（ ＭＰＩＦＡ） 试剂盒检测１００ 例呼吸道与肺部感染患儿及７６ 名健康体检儿童。结果：呼吸道感染患儿ＭＰＩｇＭ、ＩｇＣ 抗体阳性率分别为２３０ ％ 和１３０５ ，双阳性率６０ ％ ，总阳性率３００ ％ ，健康对照组儿童ＭＰＩｇＣ 阳性率５２２ ％ 。对确诊为ＭＰ阳性的２５ 例住院患儿临床资料分析；表现多样化，８０ ％病例具有持续性咳嗽，４８ ％ 有中等度发热，白细胞多在正常值范围内，Ｘ 线７２ ％ 呈间质性肺炎改变。较肺部体征出现早、临床上与其它病毒引起的呼吸道与肺部感染难以区别。双荧光染色法检测ＭＰＩｇＭ、ＩｇＣ 抗体，具有特异性强、灵敏、简便、快速的优点，为临床诊断。

case with respiratory tract infection and penumonia as well as 76 health children's serum were tested by MPIFA which was from American zeus scientific Inc. Results show that positive rate of MPIgM, IgG in respiratory tract infection was 23% and l3% res pectively, both positive rate is 6.0%,total positive rate was 30%;positive rate of MPIgG in Health children was 5.22%. The infomtation of 25 MP identited by laboratory and clinic in hospital was analyzed.The aystem were various. 80% of case had persistent cough, 48% had fever, the white blood cell in most case were in nomal level.72% case had pneumocystis by X ray, which show early then signs of lung.In clinic it not easy find the diffents from other repiratory virus infection and pneumonia diserse. The used in clinic identited that method is special, sensitive, easy and fast. It can early and fast diagnose MP. It offers a fast serum method for MP clinic diagnose and epidemiology investigation.