固定化微生物去除地下水中氯苯研究

Biodegradation of Cholrobenzene-contaminated Ground Water by Immobilized Microorganism

为探索固定化微生物技术去除地下水氯苯的最佳条件,采用聚乙烯醇(PVA)和海藻酸钠为包埋剂,培养了含氯苯的菌泥驯化培养的微生物,以制备固定化微生物小球,处理地下水中的氯苯。本研究从机械强度,传质性,氯苯降解率等方面综合考虑,利用正交实验确定了制备固定化微生物小球的最佳条件,并对固定化微生物和游离微生物降解氯苯的效果进行了比较。另外,还对固定化微生物降解地下水中氯苯的影响因素进行了探讨。实验结果表明,氯苯初始浓度大于20mg/L,固定化微生物降解氯苯效果好于游离微生物的。当小球粒径为1mm,菌液接种量为8%,氯苯初始浓度为80mg/L,pH值为7.0左右,盐度低于1.5%,控制培养温度为10℃,摇床转速为120r/min时,固定化微生物降解性能较好。

To study the optimal conditions for the biodegradation of cholrobenzene（CB）-contaminated ground water by immobilized microorganism,the CB-degrading bacteria are screened out from the CB-contaminated soil. Polyving alcohol （PVA） and sodium alginate are used to encapsulate microorganisms to make immobilized bacteria beads. The optimal conditions of the immobilized beads were identified by the orthogonal experiment,with the effect factors including mechanical strength,penetrability and the removal efficiency of the CB. The factors influencing the immobilized microorganism degrading ability were studied. The results show that the removal efficiency of the immobilized beads is better than the free microorganism. When the immobilized beads are in the optomal condition with the diameter of the bead being 1mm,the concentration of bacterial suspension 8%,the initial concentration of CB 80mg/L,pH value about 7.0,the concentration of the NaCl less than 1.5%,the temperature 10℃,and the shaking table revolution 120r/min,the biodegrading performance is quite excellent.