CsA、MPA和RPM对CD_(28)通路共刺激后淋巴细IL-4 mRNA表达的影响

The effect of CsA、MPA and RPM on IL 4 mRNA expression of CD 28 costimulated lymphocytes

目的 观察环孢素Ａ（ＣｓＡ） 、霉酚酸（ ＭＰＡ） 和雷帕霉素（ＲＰＭ） 对ＣＤ２８ 通路共刺激后淋巴细胞ＩＬ４ ｍ ＲＮＡ 表达的影响。 方法 以ＰＣＲ 扩增方法获得ＩＬ４ 特异的ｃＤＮＡ 片段，并将其克隆至ＰＧＥＭ３Ｚ 载体中。以此作为探针对ＲＴＰＣＲ 产物进行杂交定量，比较三种免疫抑制剂对ＣＤ２８通路共刺激后淋巴细胞ＩＬ４ ｍ ＲＮＡ 表达的影响。 结果 以抗ＣＤ３ ｍ Ａｂ ＋ 抗ＣＤ２８ ｍ Ａｂ 共刺激后，发现淋巴细胞稳定地表达ＩＬ４ ｍ ＲＮＡ；ＣｓＡ 和ＭＰＡ 未能抑制淋巴细胞ＩＬ４ ｍ ＲＮＡ 的表达，而ＲＰＭ则能产生显著抑制。 结论 ＲＰＭ 能抑制ＣＤ２８ 通路共刺激后ＩＬ４ ｍ ＲＮＡ 的表达，而ＣｓＡ 和ＭＰＡ对这一过程无抑制作用。

Objective To evaluate the effect of CsA、MPA and RPM on IL 4 mRNA expression of CD 28 costimulated lymphocytes. Methods A specific segment of IL 4 cDNA,obtained by means of RT PCR,was inserted into PGEM 3Z vector,and the insertion was confirmed by DNA senquencing.This probe was used to detect the level of IL 4 mRNA expression in CD 28 costimulated lymphocytes under the suppression of CsA,macophenolic acid,and rapamycin. Results It was found that IL 4 mRNA expression was markedly suppressed by rapamycin,but not by CsA and macophenolic acid. Conclusions Rapamycine has suppressive effect on this Ca 2+ independent CD 28 pathway.In this regard,rapamycine is unique to be used as an immunosuppressive drug.