摘要
目的 观察环孢素A(CsA) 、霉酚酸( MPA) 和雷帕霉素(RPM) 对CD28 通路共刺激后淋巴细胞IL4 m RNA 表达的影响。 方法 以PCR 扩增方法获得IL4 特异的cDNA 片段,并将其克隆至PGEM3Z 载体中。以此作为探针对RTPCR 产物进行杂交定量,比较三种免疫抑制剂对CD28通路共刺激后淋巴细胞IL4 m RNA 表达的影响。 结果 以抗CD3 m Ab + 抗CD28 m Ab 共刺激后,发现淋巴细胞稳定地表达IL4 m RNA;CsA 和MPA 未能抑制淋巴细胞IL4 m RNA 的表达,而RPM则能产生显著抑制。 结论 RPM 能抑制CD28 通路共刺激后IL4 m RNA 的表达,而CsA 和MPA对这一过程无抑制作用。
Objective To evaluate the effect of CsA、MPA and RPM on IL 4 mRNA expression of CD 28 costimulated lymphocytes. Methods A specific segment of IL 4 cDNA,obtained by means of RT PCR,was inserted into PGEM 3Z vector,and the insertion was confirmed by DNA senquencing.This probe was used to detect the level of IL 4 mRNA expression in CD 28 costimulated lymphocytes under the suppression of CsA,macophenolic acid,and rapamycin. Results It was found that IL 4 mRNA expression was markedly suppressed by rapamycin,but not by CsA and macophenolic acid. Conclusions Rapamycine has suppressive effect on this Ca 2+ independent CD 28 pathway.In this regard,rapamycine is unique to be used as an immunosuppressive drug.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
1999年第10期595-597,共3页
Chinese Journal of Urology
基金
国家自然科学基金
Novartis 移植科研基金