尿沉渣微卫星检测在膀胱移行细胞癌诊断中的价值

Microsatellite analysis of urine in the detection of transitional cell carcinoma of bladder

目的 探讨微卫星不稳定性（ ＭＳＩ） 检测在膀胱移行细胞癌（ＴＣＣ） 诊断中的价值。 方法 采用ＰＣＲ 扩增、聚丙烯酰胺凝胶电泳、银染等技术。选用了２０ 种微卫星标记物对５０ 例ＴＣＣ 进行分析（ 每例均包括血、尿及肿瘤组织３ 种标本） 。 结果 ５０ 例尿标本中４７ 例发生ＭＳＩ，阳性率为９４ ％ ，每例患者尿中微卫星改变均与原发肿瘤标本一致。 结论 尿沉渣ＭＳＩ检测技术是诊断膀胱肿瘤敏感有效的、无损伤的方法之一。

Objective To investigate the role of MSI in urine in TCC of bladder. Methods MSI assay was carried out for blood,urine and tumor samples of 50 cases of TCC of bladder using PCR with 20 microsatellite loci. Results MSI was positive in urine samples of 47 of 50 patients (94%) and the genetic change in urine DNA was identical to that in the primary tumor in all. Conclusions Microsatellite analysis of urine sediment is a sensitive,effective and nontraumatic means for the early detection of bladder cancer.