血液透析尿毒症患者肝炎病毒感染的研究

The study of hepatitis G virus(HGV) infection in patients on hemodialysis

目的观察尿毒症血液透析患者乙型、丙型、庚型肝炎病毒感染情况，以及此三型肝炎病毒（HCV）多重感染的情况，并探讨其感染的相对危险因素。方法对300例血液透析患者的单份血清共300份，进行HBsAb、HBsAg、HBeAg、HBeAb、HBcAb、HBV－DNA、anti－HCV、HCV－RNA的检测。并随机抽取其中的40份血清以逆转录一巢式PCR方法检测HGV－RNA，以酶联免疫吸附试验方法检测HGV抗体（anti－HGV）。结果300份血清中，HBsAg（＋）32份，HBsAb（十）97份，HBeAg（干）24份，HBeAb（＋）11份，HBcAb（＋）65份，HBV－DNA（十）20份，HBV感染率为68．67％（206／300）。anti－HCV（＋）83例，HCV－RNA（＋）56例，HCV感染率为34．67（104／300）。HBV合并HCV感染75例，占25．00％。检测其中40份血清，HGV－RNA（十）5份，anti－HGV（＋）10份，HGV感染率25．00％。HGV合并HBV感染6例（15．00％），HGV合并HCV感染5例（12．50％），HGV、HBV、HCV三重感染8例（20．00％）。结论我国亦存在庚型肝炎病毒感染，血液透析患者存在着经血行感染肝炎病毒的高危因素，开展肝炎病毒的相关研究具有重要意义。

Objective To study the infection of hepatitis G virus (HGV).hepatitis B virus (HBV) and hepatitis C virus (HCV) on hemodialysis. Methodes HBsAg、HBsAb、HBeAg、HBeAb、HBcAb、HBV-DNA.HCV-RNA.anti-HCV were measured in 300 patients on hemodialysis over 3 monthes. 40 patients of them were determined for anti-HGV by enzyme immunassay, and HGV-RNA by reverse transcription+nested polymerase chain reaction (RT-PCR). Result in 300 patients, 32 patients were positive for HBsAg, 97 patients were positive for HBsAb, 24patients were positive for HBeAg, 11 patients were positive for HBeAb, 65 patients were positivefor HBcAb, 20 patients were positive for HBV-DNA; the infection rate of HBV was 68. 67% (206/300). 83 patients were positive for anti-HCV, 56 patients were positive for HCV-RNA; the infection rate of HCV was 34. 67% (104/300). 75 (25. 00%) patients were positive for both HBV and HCV. In 40 patients 10(25. 00%) patient were positive for anti-HGV, among them 5 cases were detected HGV-RNA positive. 6 (15.00%) patients were positive for HGV and HBV; 5 (12.50%) Patients were positive for HGV and HCV. 8 patients were positive for all of HBV.HCV and HGV. Conclusions HGV infection exists in patients on hemodialysis. It is very important to study risk factors that are related to HGV.