子宫内膜癌组织中PTEN及PI3K的表达及临床意义

Expressions of PTEN and PI3K in endometrial carcinoma tissues and their clinical significances

目的:应用组织芯片技术探讨PTEN及PI3K表达与子宫内膜癌临床病理特征之间的关系及其表达相关性。方法:收集62例子宫内膜病理标本,应用组织芯片技术及免疫组化法检测PTEN及PI3K的表达。结果:PTEN和PI3K在子宫内膜良、恶性组织中阳性表达率相比,差异有统计学意义;在子宫内膜癌高中分化组与低分化组中阳性表达率相比,差异有统计学意义;在无肌层浸润或浸润深度≤1/2组与浸润深度>1/2组中阳性表达率相比,差异有统计学意义;PI3K在无淋巴结转移的子宫内膜癌中及有转移者中阳性表达率相比,差异有统计学意义;PTEN阳性表达率与PI3K阳性表达率呈负相关(γ=-0.631,P<0.05)。结论:PTEN阳性表达与子宫内膜癌组织学分级及肿瘤侵袭性呈负相关;PI3K阳性表达与子宫内膜癌组织学分级、肿瘤侵袭性及淋巴结转移呈正相关;二者在子宫内膜癌发生和发展中呈拮抗作用,表达呈负相关。组织芯片用于多因素比较研究具有高效率、质控好的优点,是一种可靠、快速、经济的高通量分析工具。监测PTEN和PI3K表达,对判断肿瘤恶性程度有重要意义。

Objective：To explore the expressions of PTEN and PI3K and the relationship with clinicopathological characteristics of endometrial carcinoma by tissue microarray technology.Methods：The pathological specimens of endometrium were collected from 62 cases,tissue microarray technology and immunohistochemical method were used to detect the expressions of PTEN and PI3K.Results：There was significant difference in positive rates of PTEN and PI3K between endometrial benign tissues and endometrial malignant tissues;there was also significant difference in positive rates of PTEN and PI3K between well/moderately differentiated endometrial carcinoma and poorly differentiated endometrial carcinoma;there was also significant difference in positive rates of PTEN and PI3K between endometrial carcinoma without myometrium invasion or the depth of myometrium invasion≤1/2 and endometrial carcinoma with the depth of myometrium invasion1/2;there was significant difference in positive rate of PI3K between endometrial carcinoma without lymph node metastasis and endometrial carcinoma with lymph node metastasis;there was a negative correlation between positive rate of PTEN and positive rate of PI3K（γ=-0.631,P0.05）.Conclusion：There is a negative correlation between positive rate of PTEN and histological grades,invasiveness of endometrial carcinoma;there is a positive correlation between positive rate of PI3K and histological grades,invasiveness of endometrial carcinoma,lymph node metastasis;there is a negative correlation between positive rate of PTEN and positive rate of PI3K,showing an antagonistic effect;tissue microarray technology has the advantages of high efficiency and good quality control in multivariate comparative study,which is a reliable,fast and economical high throughput analysis tool;monitoring the expressions of PTEN and PI3K plays an important role in predicting the degree of malignancy of endometrial carcinoma.