TRAIL对人乳腺癌、宫颈癌、卵巢癌细胞抗肿瘤作用的实验研究

Experimental study on anti-tumor effect of TRAIL on human breast cancer,cervical carcinoma and ovarian carcinoma

目的:探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)对人乳腺癌(MCF-7)、宫颈癌(Hela)、卵巢癌(SKOV-3)细胞体外抗肿瘤的杀伤作用。方法:通过MTT法、流式细胞术检测重组人TRAIL蛋白对3种肿瘤细胞和人胚肾(HEK-293)正常细胞系的作用和诱导凋亡情况。结果:一定浓度的TRAIL可有效抑制MCF-7、SKOV-3、Hela细胞,其生长抑制呈剂量依赖。TRAIL对SKOV-3细胞最敏感,用2.5 mg/L TRAIL作用SKOV-3,12h后发生凋亡,其细胞凋亡率呈时间依赖。结论:重组人TRAIL对人乳腺癌、宫颈癌、卵巢癌细胞系具有生长抑制作用,并能够诱导SKOV-3细胞凋亡。

Objective：To explore the anti-tumor effect of tumor necrosis factor-related apoptosis-inducing ligand（TRAIL） on human breast cancer（MCF-7 cells）,cervical carcinoma（HeLa cells） and ovarian carcinoma（SKOV-3 cells）.Methods：MTT assay and flow cytometry were used to detect the effect of recombination human TRAIL protein on 3 kinds of tumor cells and human embryo kidney normal cell line（HEK-293 cells） and apoptosis-inducing condition.Results：TRAIL of certain concentration could inhibit MCF-7,SKOV-3 and HeLa cells effectively,the growth inhibition showed a dose-dependent manner;TRAIL was the most sensitive to SKOV-3 cells,after treating SKOV-3 cells with 2.5 mg/L TRAIL,apoptosis occurred after 12 hours,the apoptosis rate showed a time-dependence manner.Conclusion：Recombination human TRAIL protein can inhibit the growth of human breast cancer,cervical carcinoma and ovarian carcinoma cell lines,which can induce the apoptosis of SKOV-3 cells.