口腔鳞癌细胞DNA含量分析及其临床意义

DNA Content Analysis on Squamous Cell Carcinomas of Oral Cavity and Its Clinical Significance

应用流式细胞仪对５２例口腔鳞状细胞癌石蜡标本进行ＤＮＡ含量测定，分析肿瘤细胞ＤＮＡ倍体的变化、合成期细胞百分比与临床病理指标、颈淋巴结转移、患者５年存活率的关系。结果表明：（１）二倍体及近二倍体肿瘤２７例占５６．２％（２７／４８），异倍体肿瘤２１例占４３．７％（２１／４８）。（２）合成期细胞百分比（ＳＰＦ）均值＝２０．６（９９０．９／４８），高于ＳＰＦ均值的２２例占４５．８％（２２／４８）。（３）肿瘤的倍体性、合成期细胞百分比与患者５年存活率、病理分级、颈淋巴结转移有显著关系。肿瘤倍体性、ＳＰＦ可反映肿瘤细胞的增殖活性和侵袭能力，可作为评估口腔鳞癌患者预后的指标。

Flow cytometer was used to measure the DNA content in 52 paraffin-embedded specimens of squamous cell carcinoma of oral cavity. DNA diploid variation of tumor cell was separately analyzed, the relationship between synthesis phase fraction (SPF)and clinical pathological lymphnode metastasis and 5-year survival were separately analyzed. It was shown that(1) DNA diploidy/about diploidy in 27 tumors which accounted for 43. 7% in total. (2)Average mean of SPF was 20. 6(990. 9/ 48), high SPF average mean was observed in 22 cases which accounted for 45. 8% in total. (3) Similar relationship was found between DNA diploidy SPF and 5-year survival, histopathological grading and lymphnode metastasis. The DNA diploidy and SPF reflected proliferative activity and invasivenes of tumors and that SPF can be used as an independent factor for evaluating the prognosis of the patients.