郫县豆瓣原料蚕豆中黄曲霉毒素B_1的提取

Detection of aflatoxin B_1 from raw material bean of Pixian soybean paste

郫县豆瓣中所用蚕豆是产自四川省和云南省的优质干蚕豆,经筛选、浸泡、接种、制曲等一系列加工后形成调味豆瓣酱。采用酶联免疫吸附法(ELISA),根据Box-Behnken Design软件的设计原理,检测原料蚕豆中黄曲霉毒素B1。探讨了甲醇浓度、料液比、超声波提取时间等因素对样品中黄曲霉毒素B1提取的影响,以确定检测原料蚕豆中黄曲霉毒素B1含量的最优方案。以黄曲霉毒素B1含量为响应值,利用响应面分析法对提取参数进行优化。结果表明:甲醇浓度43.41%,料液比1∶5.35,超声波提取时间39.89 min是检测原料蚕豆中黄曲霉毒素B1含量的最优条件,以该优化条件检测原料蚕豆中黄曲霉毒素B1其值达到最大,为1.50μg/kg。

Bean used in Pixian soybean paste are produced in Sichuan Province and Yunnan Province, thick broad-bean sauce is produced by screening, dipping, vaccination and a series of process. In this paper, according to the Box-Behnken Desigh software,we used the enzyme-linked immuneosorbent assay （ELISA） to test the valve of aflatoxin B1 in raw material of soybean. Taking over the influence about methanol concentration, ratio of material to solvent and ultrasonic time on the extraction of aflatoxin B1 in the samples to determine the best proposal. In addition, setting the aflatoxin B1 content as response value ,and using response surface methodology to optimize the extraction parameters. Results show that. if methanol concentration is 43.41%, ratio of material to solvent is 1 ： 5.35, ultrasonic time is 39.89 min, by this optimized condition, detecting raw material of soybean can achieve its maximum value for 1.50 μg/kg.