转Bt基因稻谷对小鼠生理与生殖的影响

Reproductive Toxicity Test of Bt Transgenic Rice on Musculus

目的:通过对小鼠饲以Bt转基因稻谷,观察Bt转基因稻谷对小鼠的生理与生殖能力的影响,为评价Bt转基因稻谷的安全性提供科学依据。方法:将雌、雄昆明小鼠48只分开饲养,各按体重随机分为转基因稻谷组和非转基因稻谷组,饲以对应的稻谷进行90天喂养试验,检测相关生理与生殖指标。结果:与非转基因稻谷组相比,饲以Bt转基因稻谷组小鼠的行为、脏器系数、血常规与血生化及怀孕母体的体重增长情况、胚胎称重、着床率、活胎数、死胎数、吸收胎数、胎儿内脏及骨骼检查等各项检测指标均无明显变化,同对照组比较差异无统计学意义。结论:Bt转基因稻谷对小鼠表观体症、脏器系数、血常规与血生化无明显不良影响。Bt转基因稻谷在本试验所用剂量和周期内供试小鼠的胚胎无致畸现象。由此可见,在本试验条件下Bt转基因稻谷对小鼠无生理和生殖毒性作用。

Objective ： In order to evaluate Bt transgenic rice＇ s security, mice were fed with transgenic Bt rice to observe the reproductive toxic effects of it. Methods：48 male and female Kunming mice were feeding separately, each group were randomly divided into the transgenic rice group and non-transgenic rice group, they were correspondingly fed for 90 days, and then combined the male and female according to a proportion of 1 ： 1 , female vaginal smears were detected on a toxicity test. Results：compared with non-transgenic rice group, Bt group＇s behavior, organ coefficient, blood and blood biochemical index had no significant adverse effects on the pregnant daily basis, the day of sperm for pregnancy 0 d and at 20 d gestation was recorded, pregnant mice were sacrificed for an autopsy. All mice were sacrificed for physiological testing after the Reproductivemother＇ s body weight, growth of the embryo weighing, implantation rate, number of live births, stillbirths number, absorption of fetal number, fetal organs, and bone scan, and other test param- eters were not significantly changed. Conclusion There is the evidence to confirm that Bt transgenic rice has adverse effects on mice＇ s physiology and fertility.