小鼠增龄过程中耳蜗MeCP2的变化

Changes of MeCP2 in the Cochlea of BALB/c Mice during the Aging Process

在对不同月龄BALB/c小鼠听功能和耳蜗形态学研究的基础上,应用免疫组化技术,检测不同月龄组(3,6,12,18月龄)小鼠耳蜗组织中MeCP2的表达与分布情况,初步探讨其在听觉老化中的作用。结果显示,MeCP2蛋白在BALB/c小鼠耳蜗中有表达,主要为细胞核着色,并随月龄增长,小鼠耳蜗染色阳性程度变弱,阳性细胞比例减少;MeCP2表达降低程度与老化程度相一致。MeCP2在小鼠耳蜗组织中的表达呈增龄相关性降低,可能与听觉老化相关。

Based on the previous studies of auditory tunction measurement and cochlea/ morphological evaluation in BALB/c mice of different age, the expression and distribution of methyl-CpG-binding protein 2 （MeCP2） in the cochlea of BALB/e mice were identified and compared among four age groups （3-, 6-, 12- and 18-month- old） by the immunohistoehemieal technique, which threw light on the effects of MeCP2 in the aging process of auditory system. The expression of MeCP2 was detected in tile immunohistochemieal staining study, predominantly in the cell nuclei. With monthly added age of the mice the positive staining was fimnd to be weaker and the proportion of the positive cells was lower. The decrease of the MeCP2 expression level in the cochlea was correlated with the degree of aging, which indicates that MeCP2 may play an important role in the aging process of auditory system.