拟南芥赤霉素受体AtGID1a原核表达条件的优化研究

Optimization for Prokaryotic Expression Conditions of Gibberellins Receptor AtGID1a in Arabidopsis

运用均匀设计法,对影响Rossetta(DE3)/pGEX-KG/AtGID1a表达水平的因素进行了优化,摸索出了对可溶性蛋白进行诱导表达的最优条件,即蛋白胨16g/L,酵母粉5g/L,葡萄糖4g/L,IPTG终浓度0.1mmol/L,诱导温度23℃,IPTG诱导时间4h。在此条件下,目的蛋白的平均密度从0.042提高到0.064,优化率为52%。

The factors that influenced expression level of Rossetta（ DE3 ）/pGEX-KG/AtG1Dla were optimized using unifotm design. The optimization induction condition for the solubility protein expression were investigated. The results were as followed： 16 g/L peptone, 5 g/L yeast powder, 4 g/L glucose, 0.1 mmol/L IPTG, 23℃ induction temperature and 4 h induction time. The average density of interest protein had increased from 0. 042 to 0. 064. The optimization rate was 52 %.