晚期糖基化终末产物荧光光谱检测法在糖尿病筛查中的应用研究

Study on the Application of Fluorescence Spectrum of Advanced Glycation End Products to Diagnosis of Diabetes Mellitus

主要研究人体皮肤晚期糖基化终末产物(Advanced glycation end products,AGE)荧光光谱的检测方法,并对AGE荧光光谱在糖尿病检测中的应用价值进行评估。利用研制的AGE荧光光谱检测装置,分别对73例受试者前臂内侧皮肤组织中AGE的荧光进行检测。同时,采用酶联免疫吸附法(ELISA)对受试者血清中的AGE水平进行检测,并利用ROC曲线分别对这两种方法的检测结果进行对比分析。结果表明,AGE荧光光谱法检测糖尿病的最佳诊断阂值为1.919AU,该点对应的敏感性为80.0%,特异性为70.4%。在特异性水平相同的情况下,荧光光谱法的敏感性优于ELISA法。AGE荧光光谱检测法具有无创、快速且受试者更容易接受等优点,在糖尿病筛查中具有重要的应用价值。

Fluorescence spectrum of the advanced glycation end products （AGE） in human skin is detected, and the evaluation of practical application in diagnosis of diabetes is also researched. A detecting system is utilized to measure the fluorescence spectrum of AGE for 73 subjects. And also, all subjects receive serum AGE tests with ELISA. The performances of fluorescence spectroscopy and those of ELISA were evaluated for sensitivity and specificity by ROC curve. At the AGE fluorescence detection threshold （ 1. 919 AU ） , the sensitivity was 80. 0 % , and the specificity was 70.4 %. While at that speeifieity, the sensitivity advantage of the AGE fluorescence over ELISA for deteeting diabetes was statistically significant. The combination of noninvasive and convenience and easily accepted by subjects makes AGE fluorescence spectroscopy method well suited for diabetes prediction and diabetes screening.