黑莓果渣不同溶剂提取物抗氧化活性研究

Study on antioxidant activity of different solvents extracts from blackberry pomaces

黑莓果渣经不同溶剂(水、pH3水、60%乙醇、80%乙醇、95%乙醇、正丁醇、乙酸乙酯)在室温下振摇提取,得到黑莓果渣不同溶剂提取物。分别采用总抗氧化能力测定体系、DPPH·(1,1-二苯基-2-苦基肼)自由基体系、羟基自由基体系、超氧阴离子自由基体系,对黑莓果渣不同溶剂提取物总抗氧化能力、清除DPPH·、O2-·和·OH的活性进行测定,并与VC进行比较。结果表明,黑莓果渣不同溶剂提取物均有不同程度抗氧化能力;酸性溶剂提取效果优于非酸性溶剂;60%乙醇为最佳的提取溶剂,其提取物总抗氧化能力、清除DPPH·和O2-·活性均弱于VC,而清除·OH活性较强且高于VC。

The blackberry pomaces was vibrational extracted using different solvent including aqueous,aqueous（pH3）,60% ethanol,80% ethanol,95% ethanol,n butanol and ethyl acetate in room temperature,respectively. And then the different extracts were obtained. The total antioxidant activity,the ·OH antioxidant capacity,O-2· and DPPH· free radical scavenging capacity of the different extracts were determined spectrophotometrically using the total antioxidant capacity assay system,DPPH· system,hydroxyl radical system,superoxide radical system. The results showed that all the extracts from the blackberry pomaces had antioxidant activity and free radical scavenging activity. The acidic solvent had better effect than non acidic solvent. The 60% ethanol was the appropriate solvent. And the total antioxidant capacity,scavenging capacity to O-2· and DPPH· of 60% ethanol extract were all weaker than VC,but the scavenging capacity to ·OH was stronger than VC.