响应面法玉米蛋白肽锌制备条件的优化

Optimization of the preparation of corn-peptide-zinc

以玉米蛋白粉为原料,碱性蛋白酶酶解制得玉米肽,与硫酸锌螯合后,得到玉米蛋白肽锌。根据Box-Behnken的中心组合实验设计原理,运用Minitab15.0数据统计分析软件,采用三因素三水平的响应面分析法优化制备玉米蛋白肽锌的工艺。结果表明:最佳的制备条件为反应时间90min、pH为5、温度63℃、蛋白肽与锌的摩尔比为2∶1,在此条件下,螯合率为57.9%。

Corn peptide was obtained via alkali protein enzyme enzymolysis with corn gluten meal as raw material, and then chelated with ZnSO4 and corn-peptide-zinc（ CPZ） was produced.The preparation process of CPZ was optimized according to Box Behnken central composition design principle. The method of response surface analysis with 3 factors and 3 levels combined with Minitab15.0statistical data analysis software was adopted. According to the above results,the optimum preparation conditions were obtained as follows： reaction time 90min, pH5,temperature 63℃,mol ratio 2∶1.Under these conditions,the chelating rate was up to 57.9%.