高效液相色谱法测定注射用肌萎灵中人参皂苷Rg1、Re、Rb1的含量

Determination of ginsenoside Rg1,Re and Rb1 in Jiweiling freeze-dried powder by HPLC

目的：建立高效液相色谱（HPLC）法测定肌萎灵冻干粉中人参皂苷Rg1、Re、Rb1的含量的方法。方法：采用Symmetry@C18（4．6mm×250mm，5μm）色谱柱，流动相为乙腈水梯度洗脱流速1．0mL·min^-1;柱温30℃；检测波长203nm。结果：人参皂苷Rg1、Re、Rb1分别在0．96～4．80，0．84～4．20，1．164-5．820μg范围内呈良好的线性关系，3种皂苷的平均回收率分别为98．62％（RSD为2．32％），99．19％（RSD为3．14％），100．17％（RSD为2．44％）。结论：该方法简便快捷，准确可靠，为肌萎灵冻干粉的质量控制提供了依据。

OBJECTIVE To establish a method for determining ginsenoside Rgl, Re and Rbl in Jiweiling freeze-dried powder. METHODS With a Symmetry@ C18 column（250 mm× 4. 6 mm,5 μm） acetonitrile-water was used as mobile phase at a flow rate of 1.0 mL· min^-1, eluted in gradient mode and column temperature maintained at 30℃. The detection wavelength was set at 203 nm. RESULTS Ginsenoside Rgl, Re and Rbl had a good linearity relationship between 0. 96-4. 80, 0. 84-4. 20, 1. 164-5. 820μg. The average recoveries were 98.62% （RSD = 2. 32%）, 99. 19% （RSD = 3. 14% ）, 100. 17% （RSD = 2.44 % ）. CONCLUSION The method is rapid,simple and accurate,and can be used for quality control of J iweiling freeze-dried powder.