纳米沸石修饰微通道反应器内固定化酶催化的水解反应动力学

Kinetics Study of Hydrolysis Catalyzed by Immobilized Enzyme in Nanozeolite Modified Microchannel Reactors

通过在毛细管内层层组装纳米沸石并固定脂肪酶来构建纳米沸石修饰的固定化酶微反应器通道,将纳米沸石良好的生物相容性和高的酶固定能力与微反应器反应效率高和扩散传质快等优点相结合,以对硝基苯棕榈酸酯的水解作为探针反应,对该微反应器内固定化酶催化水解反应动力学进行了研究和计算,并与普通反应器内同样的反应进行比较.通过对比米氏方程参数,证实在微反应器内酶催化水解反应效率比普通反应器内提高3倍以上,并可提高酶和反应底物的亲和能力.

Nanozeolite modified microchannel reactors were constructed by assembling zeolite nanocrystals into capillary using layer-by-layer assemble method.And then lipase CRL was immobilized on the nanozeolites assembled in the microreactors.The nanozeolite modified microreactors could incorporate excellent biocaptibility and large external area of nanozeolites and high efficiency and fast mass diffusion of microreactors together.Hydrolysis of 4-nitrophenyl palmitate（4-NPP） was carried out as a probe reaction to study the hydrolysis kinetics catalyzed by immobilized CRL in the microreactors.By comparing the characteristic parameters（Km and Vmax） of Michaelis-Menten equation,it is found that efficiency of enzymatic hydrolysis in such nanozeolite modified microchannel reactors could be 3 or more times higher than that of the same reaction in conventional reactors.In addition,affinity between enzyme and substrates is also enhanced in the nanozeolite modified microchannel reactors.