复合因素致大鼠实验性动脉粥样硬化模型建立

Establishment of experimental atherosclerotic model in rats with multi-factor

目的:建立适合于心血管疾病研究的复合因素致大鼠动脉粥样硬化模型。方法:健康SD雄性大鼠,分为10组,空白对照组、高脂对照组、L-met(低)组和L-met(高)组、高脂+L-met(低)组(4、6、8周)、高脂+L-met(高)组(4、6、8周)。检测血清TC、TG、HDL,检测血浆同型半胱氨酸(Hcy)、单核细胞趋化因子1(MCP-1)和白介素6(IL-6),分离各组大鼠主动脉弓,置于10%中性福尔马林中固定,作HE染色。结果:试验后,高脂对照组和高脂+L-met(低、高)(4、6、8周)组大鼠血清TC水平均明显增高;高脂对照组和高脂+L-met(低、高)(4周)组大鼠血清TG水平均明显增高;高脂对照组、L-met(低、高)和高脂+L-met(低、高)(4、6、8周)组大鼠血浆Hcy水平均明显增高;L-met(低、高)、高脂+L-met(低)(6、8周)组和高脂+L-met(高)(4、6、8周)大鼠血浆MCP-1水平均明显增高,同空白对照组相比P<0.05。L-met(低)组和高脂+L-met(低)组(4周)大鼠可见早期动脉粥样硬化改变,L-met(高)组、高脂+L-met(低)组(6、8周)和高脂+L-met(高)组(4、6周)大鼠可见中、晚期AS病理改变,高脂+L-met(高)组(8周)大鼠则出现晚期动脉粥样硬化改变。结论:通过L-met灌胃,联合应用高脂饲料喂养的方法可建立较理想的动脉粥样硬化模型。

Objective：To establish a kind of Multi-factor of rat atherosclerosis model suitable for research of cardiovascular diseases.Methods： Healthy male SD rats were randomly divided into 10 groups according to basal TC： control group,high lipid group,L-met（L）group,L-met（H）group,high lipid ＋ L-met（L）group（4,6,8W） group,high lipid ＋ L-met（H）group（4,6,8W） group.Serum lipid and plasma Hcy,MCP-1,IL-6 were determined.Thoracic aortae were isolated and prepared for pathologic examinations.Results： The levels of serum TC in high lipid group and high lipid ＋ L-met（L,H）groups（4,6,8W） were obviously higher than those in control group（P0.05）,the levels of serum TG in high lipid group and high lipid ＋ L-met（L,H）groups（4W） higher than those in control group（P0.05）,the levels of plasma Hcy in high lipid group and all experimental groups higher than those in control group（P0.05）,the levels of plasma MCP-1 in high lipid group and all experimental groups except for high lipid ＋ L-met（L）groups（4W） group higher than those in control group（P0.05）.Pathologic examinations showed that there were early aortae atherosclerotic changes in L-met（L）group and high lipid ＋ L-met（L）groups（4W）;middle and later atherosclerotic changes in L-met（H）group and high lipid ＋ L-met（L）groups（6,8W） and high lipid ＋ L-met（H）groups（4,6W）;later atherosclerotic changes in high lipid ＋ L-met（H）groups（8W） groups.Conclusion： Ideal atherosclerotic model in rats can be established by treating rats with L-met accompanied by an high lipid diet.