656例乙肝病毒检测与肝损伤的相关性分析

656 cases of liver injury in hepatitis B virus detection and analysis of the correlation

目的:探讨乙型肝炎患者病毒标志物的水平与肝损伤的相关关系。方法:丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)用连续监测法,白蛋白(ALB)用溴甲酚绿法,总胆红素(T-BIL)用矾酸氧化法,C反应蛋白(CRP)用免疫散射比浊法,乙肝五项(HBsAg、HBsAb、HBeAg、HBeAb、HBcAb)用时间分辨荧光免疫分析法,乙肝病毒DNA(HBV-DNA)用实时荧光定量PCR法。结果:不区分炎症活动期乙肝病毒标志物与肝损伤之间无明显的相关关系(均P>0.05);炎症活动期乙肝病毒标志物水平与肝损伤有一定的相关关系(P<0.05)。结论:乙肝炎症活动期定量检测乙肝病毒标志物和HBV-DNA水平,对评价乙肝患者肝细胞损伤程度有一定的意义。

Objective：To investigate the correlation of level of hepatitis B virus markers and liver injury.Methods： Alanine aminotransferase（ALT）,aspartate aminotransferase（AST） were detected with continuous monitoring method,albumin（ALB） with bromocresol green method,total bilirubin（T-BIL） with alum acid oxidation,C-reactive protein（CRP） by immune nephelometry.Five hepatitis B（HBsAg,HBsAb,HBeAg,HBeAb,HBcAb） were measured by time-resolved fluorescence immunoassay,hepatitis B virus DNA（HBV-DNA） by real-time fluorescence quantitative PCR.Results： There was no significant correlation between non-inflammatory activity of hepatitis B virus markers and liver injury（all P0.05）.However,inflammatory activity markers of hepatitis B virus and liver damage have a certain correlation（P0.05）.Conclusion： The quantitative detection of hepatitis B virus markers and HBV-DNA level during inflammatory activity period has a certain significance for the evaluation of liver cell damage in patients with hepatitis B.