婴幼儿急性下呼吸道感染和支气管哮喘抗肺炎支原体、肺炎衣原体和RSV抗体检测分析

Analysis on detection of anti-mycoplasma pneumoniae antibody,anti-chlamydia pneumoniae antibody and anti-RSV antibody in infants with acute lower respiratory tract infection and bronchial asthma

目的：了解婴幼儿急性下呼吸道感染和支气管哮喘抗肺炎支原体、肺炎衣原体和RSV抗体水平。方法：采用回顾性分析方法,将接受间接免疫荧光法检测的急性下呼吸道感染和支气管哮喘的婴幼儿患儿120例和对照组100例无相关呼吸道疾病婴幼儿的血清抗肺炎支原体（MP）抗体、抗肺炎衣原体（CP）抗体和抗呼吸道合胞病毒（RSV）抗体检测结果进行对比分析。结果：①观察组血清抗MP抗体阳性率最高,为35.83%,抗CP抗体阳性率最低,为22.50%,与对照组相比差异有统计学意义（χ^2=14.78,P〈0.05;χ^2=7.22,P〈0.05）。②婴幼儿患儿各年龄段中MP IgM、CP IgM和RSV IgM分布情况,血清MP IgM和CP IgM阳性率在13岁组最高,RSVIgM阳性率30天1岁组中最高。③抗RSV抗体在支气管哮喘中阳性率最高分别为55.56%,抗MP抗体阳性率在非典型肺炎中最高,为46.27%。④观察组患儿MP抗体、抗CP抗体和抗RSV抗体检测,单项指标的临床诊断实验评价抗MP抗体的敏感性和真实性最高,分别为37.66%和54.00%。结论：抗MP抗体、抗CP抗体和抗RSV抗体的检测是诊断婴幼儿急性下呼吸道感染和支气管哮喘中重要的参考指标。

Objective：To understand the levels of anti-mycoplasma pneumoniae antibody,anti-chlamydia pneumoniae antibody and anti-respiratory syncytial virus（RSV） antibody in infants with acute lower respiratory tract infection and bronchial asthma.Methods：A retrospective analysis method was used in the study;the detection results of serum anti-mycoplasma pneumoniae antibody,anti-chlamydia pneumoniae antibody and anti-RSV antibody in 120 infants with acute lower respiratory tract infection and bronchial asthma and 100 infants without corresponding respiratory diseases were compared and analyzed by indirect immunofluorescence detection.Results：The positive rate of serum anti-mycoplasma pneumoniae antibody in observation group was the highest（35.83%）,the positive rate of serum anti-chlamydia pneumoniae antibody was the lowest（22.50%）,compared with control group,there was significant difference（χ^2=14.78,P〈0.05;χ^2=7.22,P〈0.05）;the distributions of mycoplasma pneumoniae IgM,chlamydia pneumoniae IgM and RSV IgM in infants of all age groups： the positive rates of mycoplasma pneumoniae IgM and chlamydia pneumoniae IgM in 13 year-old group was the highest,the positive rate of RSV IgM in 30 days1 year group was the highest;the positive rate of anti-RSV antibody in infants with bronchial asthma was the highest（55.56%）,the positive rate of anti-mycoplasma pneumoniae antibody in infants with atypical pneumonia was the highest（46.27%）;the susceptibility and authenticity of anti-mycoplasma pneumoniae antibody detection were the highest（37.66% and 54.00%,respectively）.Conclusion：Anti-mycoplasma pneumoniae antibody,anti-chlamydia pneumoniae antibody and anti-RSV antibody are reference indexes of diagnosis of infants with acute lower respiratory tract infection and bronchial asthma,which play important role in etiological diagnosis and treatment