大鼠梗阻性黄疸脑内多胺代谢紊乱的实验研究

Metabolic disorder of polyamine in obstructive jaundice of rats

目的研究梗阻性黄疸大鼠脑组织多胺代谢变化及临床意义。方法雄性Wistar大鼠26只，随机分为假手术组（SO组）和胆总管结扎组（BDL组）。术后9d门静脉抽血测量血清总胆红素（TBil）水平；取大鼠脑组织，高效液相色谱（HPLC）法测定腐胺、精脒和精胺的含量；比色法测定脑组织多胺氧化酶（PAO）活性水平。结果BDL组大鼠与SO组比较，血清TBil明显升高（P〈0．05），脑组织腐胺水平明显升高（P〈0．05），而精脒、精胺水平明显下降（P〈0．05），PAO活性水平明显升高（P〈0．05）。结论梗阻性黄疸大鼠脑内多种多胺成分代谢水平发生明显改变，可能参与了胆汁淤积性脑病的发病机制。

Objective To study the metabolic changes of polyamine in brain tissue of rats with obstructive jaundice. Methods Twenty-six male Wistar rats were randomly divided into two groups as sham operation（SO）and bile duct ligation（BDL）. On the 9th day levels of serum total bilirubin level （TBil） in portal vein were determined. The brain tissues of rats were collected, and the contents of putrescine, spermidine and permine were measured by high-performance liquid chromatography（HPLC） method. Colorimetric method was applied to examine the activity of polyamine oxidase（PAO）in the brain tissue of rats. Results As compared with SO group,the levels of serum total bilirubin in portal vein of rats in BDL group were significantly increased（P〈0. 05） ;putrescine content was significantly increased, but contents of sperrnidine and permine were decreased in BDL group（all, P〈0. （15）;The activity of PAO was significantly increased in BDL group（P〈0. 05）. Conclusion The metabolism of polyamine changes significantly in BDL groups, which may take part in the pathogenesis of cholestatic encephalopathy in obstructive jaundice.