两种材料复合rhBMP-2诱导大鼠皮下异位成骨的比较研究

Subcutaneous ectopic osteogenesis induced by porous calcium phosphate cement and gelatin sponge as the carrier of recombinant bone morphogenetic protein-2 in rats: A comparative study

目的分析多孔自固化磷酸钙骨水泥(Calcium Phosphate Cement,CPC)和明胶海绵复合重组人骨形态发生蛋白(Recombinantion Humen Bone Morphogenetic Protein-2,rhBMP-2)诱导大鼠皮下异位成骨的区别。方法平均质量200g SD大鼠30只,麻醉后分别植入A:多孔CPC复合rhBMP-2(2μg);B:多孔CPC;C:明胶海绵复合rhBMP-2(2μg);D:空白明胶海绵,无菌喂养后分别于2、4、8周各处死10只,对植入部位组织取材,分别进行micro-CT扫描,并使用Micview V2.1三维重建处理软件扫及ABA骨形态分析软件检测,记录组织骨密度(Tissue Mineral Density,TMD)及骨小梁厚度(Trabecular Thickness,Tb.Th)。运用SPSS10.0统计软件进行统计学分析。后行甲醛固定2周,石蜡包埋切片,HE染色进行组织学观察。结果在2、4、8周时,加入rhBMP-2的两实验组表现更高的组织骨密度和骨小梁厚度,且各组数据随时间递增,其差异有统计学意义。结论多孔磷酸钙骨水泥可以作为rhBMP-2的一种良好载体保证其发挥成骨作用。

Objective To analyze the difference in subcutaneous ectopic osteogenesis induced by porous calcium phosphate cement(CPC) and gelatin sponge as a carrier of recombinant bone morphogenetic protein-2(rhBMP-2).Methods Thirty Sprague Dawley rats with an average body weight of 200g were divided into groups A-D.CPC+rhBMP-2,CPC,gelatin sponge+rhBMP-2,and gelatin sponge were implanted into the rats after anesthesia.Ten rats were killed 2,4 and 8 weeks after they were fed under sterile environment.Bone tissue samples were collected from the implantation sites.Tissue mineral density(TMD) and trabecular thickness were detected with micro-CT scanner and analyzed with SPSS10.0 statistical software.Bone tissue was fixed in 4% paraformaldehyde for 2 days,embedded in paraffin,and cut into sections.The sections were stained with H&E to observe their histological change.Results The tissue mineral density and trabecular thickness of the samples with rhBMP-2 were higher in two experimental groups 2,4 and 8 weeks after implantation,which increased with the prolongation of time(P<0.05).Conclusion Porous CPC can be used as a carrier of rhBMP-2 for osteogenesis.