参龙健脑胶囊对血管性痴呆大鼠海马蛋白质表达的影响

Effect of shenlongjiannao capsules on expression of hippocampal proteins in rats with vascular dementia

目的探讨参龙健脑胶囊对血管性痴呆模型大鼠海马蛋白质表达的影响。方法采用双侧颈总动脉结扎的方法制备血管性痴呆模型,30只大鼠分为模型组,参龙健脑胶囊组和假手术组,给药4周后进行Morris水迷宫实验并应用双向凝胶电泳和质谱鉴定观察参龙健脑胶囊对血管性痴呆大鼠海马蛋白表达的影响。结果参龙健脑胶囊能提高模型大鼠的学习记忆能力,同时参龙健脑胶囊组与模型组相比有7个蛋白下调,2个蛋白上调,经质谱检测最终得到8种蛋白质。结论参龙健脑胶囊可通过影响血管性痴呆模型大鼠海马蛋白质表达而提高其学习记忆能力。

Objective To study the effect of shenlongjiannao capsules on expression of hippocampal proteins in rats with vascular dementia（VD）.Methods A rat VD model was established by ligating the bilateral common carotid arteries.Thirty rats were randomly divided into model group,shenlongjiannao capsule group and sham operation group.Morris water maze test was performed after the rats had been given shenlongjiannao capsules for 4 weeks and the differences of hippocampal proteins between model group and shenlongjiannao capsule group were analyzed by two-dimensional polyacryalmide gel electrophoresis and ultraflex TOF/TOF mass spectrography.Results Shenlongjiannao capsules could significantly improve the learning and memory ability of model rats.The expression of 7 and 2 proteins was down-regulated and up-regulated,respectively,in shenlongjiannao capsule group compared to that in model group.Eight proteins were identified by ultraflex TOF/TOF mass spectrography.Conclusion Shenlongjiannao capsules can improve the learning and memory ability of rats with VD by regulating the expression of hippocampal proteins.