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胃腺癌组织中cFLIP mRNA的表达及细胞凋亡检测 被引量：1

Detection of cFLIP mRNA and cell apoptosis in gastric adenocarcinoma tissue
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摘要 目的:检测胃腺癌组织中cFLIP mRNA的表达及胃腺癌细胞的凋亡。方法:采用原位杂交技术检测45例胃腺癌、23例癌旁不典型增生及21例正常胃黏膜组织中cFLIP mRNA的表达;运用TUNEL法检测胃腺癌细胞凋亡情况,计算凋亡指数(AI)。结果:胃腺癌组织中cFLIP mRNA的阳性表达率高于正常黏膜及不典型增生组织(χ2=5.818和4.900,P=0.012和0.021)。胃腺癌组织中cFLIP mRNA的表达与其分化程度、浸润深度和淋巴结转移有关(χ2=6.171,16.291和6.799,P均<0.05)。胃腺癌组织中cFLIP mRNA阳性表达组AI值明显低于阴性表达组(t=7.425,P<0.001)。结论:cFLIP与胃腺癌的发生发展和浸润转移密切相关,并可能在抑制胃腺癌细胞凋亡中发挥重要作用。 Aim：To detect the expression of cFLIP mRNA and apoptosis in gastric adenocarcinoma tissue.Methods：The expression of cFLIP mRNA in 45 cases of gastric adenocarcinoma,23 cases of gastric corresponding atypical hyperplasia,and 21 cases of normal gastric tissue was detected by using in situ hybridization.The apoptosis was examined by TUNEL assay.Results：Compared with the gastric adenocarcinoma group,the positive expression rates of cFLIP mRNA in normal gastric tissue and atypical hyperplasia tissue both decreased（χ^2=5.818 and 4.900,P=0.012 and 0.021）.In gastric adenocarcinoma tissue,AI in the tissue with positive expression of cFLIP mRNA was lower than that with negative expression（t=7.425,P0.001）.Conclusion：cFLIP is closely related to the carcinogenesis,development,infiltration and metastasis of gastric adenocarcinoma and may play an important role in the inhibition of gastric adenocarcinoma cell apoptosis.
作者 沈新生 张谢夫 陈奎生 薛艳格
机构地区 郑州大学第一附属医院普通外科 郑州大学第一附属医院病理科
出处 《郑州大学学报（医学版）》 CAS 北大核心 2011年第1期42-44,共3页 Journal of Zhengzhou University(Medical Sciences)
关键词 胃腺癌 CFLIP 凋亡 gastric adenocarcinoma cFLIP apoptosis
分类号 R735.2 [医药卫生—肿瘤]
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参考文献10

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二级参考文献23

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郑州大学学报（医学版）
2011年 第1期

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