恶性黑色素瘤淋巴道转移裸小鼠模型的建立

Establishment of malignant melanoma lymphatic metastasis model in nude mice

目的:建立恶性黑色素瘤淋巴道转移裸小鼠模型。方法:12只裸小鼠分为2组,每组6只。实验组通过裸小鼠角膜囊袋植入恶性黑色素瘤A375细胞建立恶性黑色素瘤淋巴道转移裸小鼠模型,对照组未植入瘤细胞。2周后取材,进行淋巴内皮细胞特异性抗体LYVE-1角膜全组织荧光染色,采用图像分析法测定角膜淋巴管总体面积,RT-PCR法检测角膜组织中血管内皮生长因子C(VEGF-C)mRNA的表达。结果:实验组小鼠角膜淋巴管总体面积[(2.168±0.124)×103μm2]较对照组[(1.053±0.032)×103μm2]增加(t=4.402,P=0.005)。实验组VEGF-CmRNA呈特异性强表达,而对照组表达极弱或不表达。结论:成功建立了恶性黑色素瘤淋巴道转移动物模型;VEGF-C可能是肿瘤诱导淋巴管新生的内源性诱因。

Aim：To establish a nude mice model of malignant melanoma lymphatic metastasis.Methods：The mice in experimental group（n=6）were implanted malignant melanoma A373 cells combined with matrigel into the cornea.The mice in control group（n=6）were given PBS combined with matrigel into the cornea.After 2 weeks,the immunorescent whole mount staining for lymphatic specific marker LYVE-1 was performed,and RT-PCR was used to detect the expression of vascular endothelial growth factor-C（VEGF-C）mRNA.Results：The total area of lymphatic vessels in expreimental group [（2.168±0.124）×10^3 μm^2] was higher than that in control group [（1.053±0.032）×103 μm2],t=4.402,P=0.005.The expression of VEGF-C mRNA in experimental group was strong,while that in control group was weak.Conclusion：The malignant melanoma lymphatic metastasis model in nude mice has been successfully established.VEGF-C is an important growth factor contributing to lymphatic metastasis.