胰岛素对βTC-3细胞胰岛素受体表达的作用

Effect of Insulin on Expression of Insulin Receptor in TC-3 Cell

目的:观察外源性胰岛素对小鼠胰岛β细胞瘤细胞株βTC-3细胞胰岛素受体表达的影响。方法:采用免疫荧光细胞化学技术结合激光扫描共聚焦显微镜观察高浓度胰岛素(100 IU/ml)刺激不同时间(0 min、30 min、60 min、120 min、240 min),培养的βTC-3细胞胰岛素受体的表达,用Image pro plus软件对胰岛素受体的荧光强度进行了半定量分析。结果:与0 min比较,胰岛素孵育30 min、60 min、120 min、240 min时胰岛素受体荧光强度均明显下降(P<0.05)。结论:高浓度胰岛素孵育βTC3细胞后,可明显下调胰岛素受体的表达,这可能是高胰岛素血症导致胰岛素抵抗产生的机制之一。

Objective： To explore the effects of exogenous insulin on the expression of insulin receptors in mouse insulinoma TC-3 cells.Methods： The expression of insulin receptors in TC-3 cells in different incubation time（0 min,30mins,60mins,120mins,240mins） exposure to 100 IU/ml insulin was analyzed by immune fluorescence cytochemistry combined with laser scanning confocal microscopy.Semi-quantitative analysis of fluorescence intensity of insulin receptors was determined by Image Pro Plus 6.0 software.Results： The expression of insulin receptors at 30,60,120 and 240 min was decreased significantly compared with that of 0 min incubated with 100 IU/ml insulin（P0.05）.Conclusion： The expression of insulin receptor in βTC3 cells was obviousiy decreased with high concen-tration insulin incubation,which may be one of the mechanism of insulin resistance caused by hyperinsulinemia.