丹参配伍三七和川芎不同提取工艺对丹参酮ⅡA含量的影响

Compatibility of Salvia Miltiorrhiza with Panax Notoginseng and Rhizome Chuanxiong: Effect of Different Extraction Technologies on Content of TanshinoneⅡA

目的:丹参分别配伍三七和川芎经水煎煮和70%乙醇回流后,检测丹参酮ⅡA含量的变化,探究配伍提取后对丹参中可检测成分含量的转移规律。方法:丹参,丹参+三七,丹参+川芎,丹参+三七+川芎分别水煎煮、70%乙醇回流提取后,用TLC和HPLC方法检测丹参酮ⅡA含量。结果:①全部水煎煮物中基本不含丹参酮ⅡA;②丹参单独醇回流和丹参+川芎混合醇回流加入三七后,丹参酮ⅡA含量分别上升16.66%和54.97%;③丹参单独醇回流和丹参+三七混合醇回流加入川芎后,丹参酮ⅡA含量分别下降57.34%和43.33%;④丹参单独醇回流加入三七+川芎后,丹参酮ⅡА含量下降33.89%;结论:丹参配伍三七显现中药的"相使"药性;而川芎对丹参则是"相恶"药性,但三七可缓解川芎对丹参的"相恶"药性。

Objective： To investigate the content change of tanshinoneⅡA in extractive for Salvia Miltiorrhiza compatibility of Panax Notoginseng and Rhizome Chuanxiong by water decoction method and 70% ethanol refluxing extraction method. Methods： The tanshinoneⅡA were extracted by water decoction method and 70% ethanol reflux method in the Salvia Miltiorrhiza group, Salvia Milti-orrhiza with Panax Notoginseng group, Salvia Miltiorrhiza with Rhizome Chuanxiong group, Salvia Miltiorrhiza with Panax Notoginseng and rhizome chuanxiong group, and the content of tanshinoneⅡA were determined by TLC and HPLC. Results： ①TanshinoneⅡA canhardly be detected in the water decoction. ②Ethanol refluxing extracted with Panax Notoginseng, the content of tanshinoneⅡA rose by16.66% and 54.97% in the Salvia Miltiorrhiza group and Salvia Miltiorrhiza with Rhizome Chuanxiong group. ③Ethanol refluxing ex-tracted with Rhizome Chuanxiong, the content of tanshinoneⅡA decreased by 57.34% and 43.33% in the Salvia Miltiorrhiza group andSalvia Miltiorrhiza with Panax Notoginseng group. ④Ethanol refluxing extracted with Panax Notoginseng and rhizome chuanxiong, thecontent of tanshinoneⅡA decreased by 33.89% in the Salvia Miltiorrhiza group. Conclusion： Pharmacological action for Salvia Miltior-rhiza compatibility of Panax Notoginseng has mutual assistance effect, compatibility of Rhizome Chuanxiong has mutual inhibitioneffect, and the mutual inhibition effect can be reduced by Panax Notoginseng.