摘要
目的比较常用的国产与进口银杏叶片中所含萜类内酯,以保证临床用药的有效性。方法采用Ultimate 3000高效液相色谱仪结合蒸发光散射检测器,Zorbax SB-C18柱(250mm×4.6mm,5μm),以正丙醇-四氢呋喃-水(1:15:84)为流动相,体积流量1.0mL/min,漂移管温度115℃,压缩气体氮气体积流量为3.00L/min,检测国产和进口2种银杏叶片各3批所含银杏内酯A、B、C和白果内酯的量,并计算内酯总量。结果 2种银杏叶片每片所含银杏内酯A、B、C和白果内酯分别为(1.29±0.05)、(0.69±0.05),(0.83±0.17)、(0.45±0.09)mg,(0.45±0.05)、(0.46±0.02),(1.38±0.15)、(1.26±0.06)mg,内酯总量分别为(3.95±0.11)、(2.87±0.22)mg。结论 2种银杏叶片所含萜类内酯的量均符合中国药典的规定,其中二者所含银杏内酯C和白果内酯的量相似,但国产银杏叶片所含银杏内酯A、B的量以及内酯总量明显高于进口银杏叶片。
Objective Comparing the contents of triterpene lactones in two kinds （domestic and imported） of Ginkgo biloba Tablets commonly used in the hospital, so as to ensure the clinical efficacy. Methods An HPLC-ELSD method was employed to determine the contents of ginkgolide A, B, C, bilobalide and the totai triterpene lactones in three lots of Chinese and imported Ginkgo biloba Tablets. The analysis was performed on Zorbax SB-C18 column （250 mm × 4.6 mm, 5 μm） with n-propanol - tetrahydrofuran - water （1 ： 15 ： 84） as mobile phase at a flow rate of 1.0 mL/min. The drift tube temperature of the ELSD Detector was 115℃ and the evaporative gas was nitrogen at speed of 3.00 L/min. Results The contents of ginkgolide A, B, C, and bilobalide in three lots of domestic and imported Ginkgo biloba Tablets were （1.29 ±0.05）, and （0.69 ± 0.05）; （0.83 ± 0.17）, and （0.45 ± 0.09） mg; （0.45 ± 0.05）, and （0.46 ± 0.02）, （1.38 ±0.15）, and （1.26 ± 0.06） mg per tablet; The total contents of lactones were （3.95 ± 0.11） and （2.87 ± 0.22） mg, respectively. Conclusion Contents of terpene lactones in domestic and imported Ginkgo biloba Tablets are both qualified in accordance with Pharmacopoeia of People＇s Republic of China; And the two kinds of tablets have similar contents ofginkgolide C and bilobalide, but the contents of ginkgolide A, B and total terpene lactones in domestic Ginkgo biloba Tablet are obviously more than those in imported Ginkgo biloba Tablet.
出处
《药物评价研究》
CAS
2011年第1期35-38,共4页
Drug Evaluation Research
基金
国家973资助项目(2005CB523400)
