竹节参多糖对环磷酰胺致免疫低下小鼠的恢复作用研究

Effects of Polysaccharides Isolated from Panax japonicus on Immunosuppression Mice

目的:研究竹节参多糖(Panax japonicus polysaccharides)对环磷酰胺所致免疫低下模型小鼠免疫功能的调节作用。方法:腹腔注射环磷酰胺(cyclophosphamide,Cy)制备免疫功能低下模型,计算脾脏指数;MTT法检测脾淋巴细胞增殖活性;分光光度法检测血清溶血素的含量;QHS法检测脾脏分泌抗体形成细胞的能力;流式细胞术检测外周血NK细胞百分比。结果:竹节参多糖能提高免疫低下小鼠的脾脏指数;显著促进脾淋巴细胞的增殖;促进血清溶血素的生成及QHS反应;提高外周血NK细胞比例。结论:竹节参多糖具有较好的免疫增强作用,对免疫低下小鼠的免疫系统具有恢复作用。

Objective ：To investigate the immunomodulatory eltects oi polysaccharldes Irom Panax japontcus on immunosuppressed mice. Methods：Immunosuppressed routine model were established in Kuming mice induced by cyclophosphamide（Cy） and splenic indexes were evaluated. The proliferation activities of splenic lymphocyte were assayed by MTF and levels of serum hemolysin were confirmed by spectrophotometry. The antibody-secreting capbilities of splenocytes were assayed by quan-titative haemolysis of SRBC （QHS）. The percentage of NK cells were analysed by flow cytometry（FCM）. Resuhs ：Polysaccharides of Panax japonicus significantly increased the splenic indexes, promoted the proliferation of splenic lymphocytes, enhanced QHS reaction, elevated the production of serum hemolysin and percentages of peripheral blood NK cells in Cy-induced immunosuppressed mice. Conclusion：Panax japonicus polysaceharides can recover immune function in the Cy-induced immunosuppressed mice.