壳聚糖、溶菌酶和牛至油对大豆分离蛋白膜抑菌效果的影响

Effects of Chitosan,Lysozyme and Oregano Oil on Antimicrobial Activity of Soy Protein Isolate Film

采用双倍稀释法研究壳聚糖、溶菌酶和牛至油对大肠杆菌和金黄色葡萄球菌的最小抑菌浓度。采用抑菌圈法研究添加天然抑菌剂壳聚糖、溶菌酶和牛至油的大豆分离蛋白膜对大肠杆菌、金黄色葡萄球菌、酿酒酵母和黑曲霉的抑菌效果。结果表明,添加壳聚糖、溶菌酶和牛至油的大豆分离蛋白膜对大肠杆菌、金黄色葡萄球菌、酿酒酵母和黑曲霉均有抑制作用。对大肠杆菌和金黄色葡萄球菌抑制效果为:牛至油>壳聚糖>溶菌酶。对酿酒酵母的抑制效果为:壳聚糖>牛至油>溶菌酶;对黑曲霉的抑制效果为:牛至油>壳聚糖>溶菌酶。因此,添加壳聚糖、溶菌酶和牛至油的大豆分离蛋白膜具有较好的抑菌效果和应用前景。

Double dilution method was used to determine minimum inhibitory concentration of chitosan, lysozyme and oregano oil against Eschvrichia coli and Staphyloccocus aureus. Inhibition zone method was used to determine antimicrobial effect of soy protein isolate films enriched with chitosan, lysozyme and oregano oil against E. coli, S. aureus, Saccharomyces cerevisiae and Aspergillus niger. Results indicated that soy protein isolate films enriched with chitosan, lysozyme and oregano oil could inhibit the growth of E. coli, S. aureus, S. cerevisiae and A. niger. The inhibitory effect on E. coli and S. aureus ： oregano oil 〉 chitosan 〉 lysozyme. The inhibitory effect on S. cerevisiae： chitosan 〉 oregano oil 〉 lysozyme; the inhibitory effect on A. niger： oregano oil 〉 chitosan 〉 lysozyme. This study showed the films en- riched with chitosan, lysozyme and oregano oil have potential to be used as active biodegradable films with strong antimicrobial effects.