提取酵母细胞壁中β-D-葡聚糖的新方法

New Method for Extraction of β-D-glucan from Yeast Cell Walls

研究了一种从酵母细胞壁中提取β-D-葡聚糖的新方法,包括高温抽提、脱脂和酶解3部分。在高温条件下分别考察了pH、料液比、温度及时间对β-D-葡聚糖得率和纯度的影响。结果表明,高温提取的最佳工艺条件为:料液比为1:15、pH=8、温度120℃,时间3 h,β-D-葡聚糖得率为61.05%,纯度达到41.51%;为了进一步提高β-D-葡聚糖的纯度,采用了脱脂和酶解的方法去除粗多糖中的蛋白质、脂质等杂质,最终产品的纯度达到78.31%。

A new process comprising hot water extraction, removal of the lipids and protease treatment was studied for the isolation of β-D-glucan from yeast cell walls in this paper. The effects of different pH, solid-liquid ratio, extraction temperature and time on extraction of β-D-glucan were investigated. The optimum process conditions were determined as following ： the solid-liquid ratio, 1 ： 15 ; pH,8 ; temperature, 120℃ ; time, 3h. The yield and purity of β-D-glucan was 61.05% and 41.51% respectively by the process. In order to improve the purity of β-D-glucan, lipid extraction and enzymatic treatment were used to remove the remaining proteins and lipids. Finally, β-D-glucan purity reached 78.31%.