葡萄籽原花青素对血管性痴呆大鼠的治疗作用研究

The protective effect of grape seed proanthocyanidin on vascular dementia in rats

目的研究葡萄籽原花青素对大鼠血管性痴呆的治疗作用。并探讨这一作用的机制。方法采用大脑中动脉栓塞法制备血管性痴呆大鼠模型，将50只SD大鼠分为假手术组、模型组、阳性对照组（银杏叶提取物片）、葡萄籽原花青素低剂量组（50mg·kg^-1）和葡萄籽原花青素高剂量组（150mg·kg^-1），每组10只。通过水迷宫法和跳台实验测定大鼠学习记忆能力，并测定脑组织内超氧化物歧化酶（SOD）和胆碱酯酶（ChE）的活性、总抗氧化能力（T—AOC）及丙二醛（MDA）含量。结果低和高剂量原花青素均可使大鼠水迷宫潜伏期明显缩短，穿台次数明显增加；延长跳台实验潜伏期，减少跳台错误总数；提高脑组织SOD活性和T—AOC。降低脂质过氧化物含量和ChE活性。结论原花青素有抗大鼠血管性痴呆的作用，作用机制与其提高机体抗氧化能力和降低脑组织内ChE活性有关。

Objective To investigate the effect of grape seed procyanidin（GSP） on vascular dementia （VD） in rats and its mechanism. Methods The vascular dementia was induced by occlusion of middle cerebral artery for 2h in rats. Fifty rats were randomly divided into five groups： sham operation group, VD group, positive control group （ginkgo biloba leaves extract）, GSP group （50mg·kg^-1） and high-dose GSP group （100mg·kg^-1）. The abilities of learning and memory in rats were measured by Morris water maze and step-down avoidance test. The contents of malondialdehyde （MDA）, superoxide dismutase （SOD）, cholinesterase （ChE）, total antioxidant capacity （T-AOC） in brain tissue of ischemic hemisphere were measured. Results GSP significantly shortened the escape latency period and increased the frequency of crossing the former platform site in Morris water maze, extended the latency and decreased the frequency of errors in step-down avoidance test. GSP increased SOD and T-AOC contents,decreased ChE activity and MDA contents in brain tissue. Conclusion GSP has protective effect on vascular dementia in rats, which is associated with its effects on anti-oxidation, scavenging oxidation free radical and decreasing ChE activity.