摘要
目的 研究细胞外信号调节激酶（ERK1/2）和蛋白激酶B（PKB/Akt）在糖尿病大鼠阴茎海绵体中的表达,探讨糖尿病大鼠勃起功能障碍（ED）可能存在的发病机制.方法 20只8周龄雄性Wistar大鼠随机分成对照组（A组）和实验组（B组）.实验组采用1%链脲佐菌素（STZ）腹腔注射建立I型糖尿病大鼠模型,对照组给予等量生理盐水腹腔注射,4周后采用免疫组化及RT-PCR技术检测ERK1/2和PKB/Akt在大鼠阴茎海绵体中的表达.结果 ERK1、ERK2的mRNA和ERK1/2蛋白的表达量在B组（0.7343±0.0820、0.7962±0.0730、0.1574±0.0350）较A组（0.3943±0.0900、0.4156±0.0590、0.1205±0.0360）显著升高,差异具统计学意义（P〈0.05）;PKB/Akt的mRNA和PKB/Akt蛋白的表达量在B组（0.9884±0.0460、0.1822±0.0470）与A组（0.9417±0.0540、0.1586±0.0220）差异无统计学意义（P〉0.05）.结论 ERK1/2和PKB/Akt在糖尿病大鼠阴茎海绵体中均有表达,糖尿病大鼠阴茎海绵体ERK1/2表达增强可能与糖尿病大鼠ED的发生有关.
Objective To analyze the expressions of ERK1/2 and PKB/Akt in corpus cavemosums of diabetic rats and explore the possible mechanism of erectile dysfimction（ED） of diabetic rats. Methods Twenty Wistar rats with 8 weeks age were randomly devided into group A（ the control group） and group B （the experimental group group）. Rats in group B were given peritoneal injection with 1% streptozocm and rats in group A were injected with equal volume 0.9% normal saline. Four weeks later, the expressions of ERK1/2 and PKB/Akt in corpus cavemosum of rats were detected by immunohistochemistry staining and RT-PCR. Results The mRNA expression of ERK1 and ERK2, as well as the protein expression of ERK1/2 in corpus cavemosum of rats in group B（0.7343±0.0820, 0.7962±0.0730 and 0.1574±0.0350） was significantly higher than that in group A（0.3943±0.0900, 0.4156±0.0590 and 0.1205±0.0360）（P〈0.05）. No significant difference was found in the mRNA and protein expression of PKB/Akt between in group B （0.9884±0.0460 and 0.1822±0.0470） and m group A（0.9417±0.0540 and 0.1586±0.0220）（P〉0.05）. Conclusion The expressions of ERK1/2 and PKB/Akt were all detected in penile tissue of diabetic rots. Whereas, high expression of ERK1/2 in corpus cavemosum might be associated with development of ED of diabetic rats.
出处
《中国男科学杂志》
CAS
CSCD
2011年第1期3-7,10,共6页
Chinese Journal of Andrology
基金
四川省科技厅基金（060045）
